Department of Zoology, University of Delhi, Delhi-110 007, India.
Int J Syst Evol Microbiol. 2011 Sep;61(Pt 9):2107-2111. doi: 10.1099/ijs.0.017624-0. Epub 2010 Sep 24.
A bacterial strain, designated P15(T), was isolated from the soil of an open hexachlorocyclohexane dumpsite. Comparative sequence analysis showed that strain P15(T) displayed high 16S rRNA gene sequence similarities (94.4-97.2 %) with members of the genus Pseudoxanthomonas. The isolate was most closely related to Pseudoxanthomonas mexicana AMX 26B(T) (97.2 % 16S rRNA gene sequence similarity) and Pseudoxanthomonas japonensis 12-3(T) (97.2 %). DNA-DNA relatedness studies showed unambiguously that strain P15(T) represented a novel species that was separate from P. mexicana DSM 17121(T) (7.7 %) and P. japonensis DSM 17109(T) (9.4 %). The predominant cellular fatty acids of strain P15(T) were iso-C₁₆:₀ (21.4 %), iso-C₁₅:₀ (16.1 %), summed feature 9 (comprising iso-C₁₇:₁ω9c and/or 10-methyl C₁₆:₀; 14.9 %), iso-C₁₁:₀ 3-OH (8.3 %) and iso-C₁₄:₀ (7.0 %). The polar lipid profile of strain P15(T) showed the presence of large amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol in addition to unknown glycolipids, phospholipids and an amino-group-containing polar lipid. Ubiquinone 8 was found as the major quinone. The polyamine profile showed the presence of spermidine. The DNA G+C content was 62.9±2 mol%. Strain P15(T) is described as representing a new member of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas indica sp. nov. is proposed. The type strain is P15(T) ( = MTCC 8596(T) = CCM 7430(T)).
一株细菌菌株，命名为 P15(T)，从一个开放的六氯环己烷倾倒场的土壤中分离得到。比较序列分析表明，菌株 P15(T)与假单胞菌属的成员具有高的 16S rRNA 基因序列相似性（94.4-97.2%）。该分离株与 Pseudoxanthomonas mexicana AMX 26B(T)（97.2% 16S rRNA 基因序列相似性）和 Pseudoxanthomonas japonensis 12-3(T)（97.2%）最为密切相关。DNA-DNA 同源性研究明确表明，菌株 P15(T)代表一个新的物种，与 Pseudoxanthomonas mexicana DSM 17121(T)（7.7%）和 Pseudoxanthomonas japonensis DSM 17109(T)（9.4%）分开。菌株 P15(T)的主要细胞脂肪酸为 iso-C₁₆:₀（21.4%）、iso-C₁₅:₀（16.1%）、总和特征 9（包括 iso-C₁₇:₁ω9c 和/或 10-甲基 C₁₆:₀；14.9%）、iso-C₁₁:₀ 3-OH（8.3%）和 iso-C₁₄:₀（7.0%）。菌株 P15(T)的极性脂谱显示存在大量的磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油，此外还有未知的糖脂、磷脂和含氨基的极性脂。发现泛醌 8 为主要的醌。聚胺谱显示存在亚精胺。DNA G+C 含量为 62.9±2 mol%。菌株 P15(T)被描述为代表假单胞菌属的一个新成员，建议将其命名为印度假单胞菌（Pseudoxanthomonas indica）。模式菌株为 P15(T)（=MTCC 8596(T)=CCM 7430(T)）。
