8型腺病毒基因组类型的限制性酶切位点图谱

Restriction site mapping of adenovirus type 8 genome types.

作者信息

Adrian T, Wolf U, Lauer H J, Wigand R

机构信息

Nationales Referenzzentrum für Adenoviren, Institut für Virologie und Seuchenhygiene, Medizinische Hochschule, Hannover, Germany.

出版信息

Res Virol. 1990 Nov-Dec;141(6):611-24. doi: 10.1016/0923-2516(90)90034-g.

DOI:10.1016/0923-2516(90)90034-g

PMID:2087599

Abstract

The DNA of 60 adenovirus type 8 (AV8) isolates (collected during the period 1961 to 1982, mostly in Western Germany) was analysed by 6 endonucleases and revealed 6 different genome types, thus implying that the variability of AV8 is relatively low. It was found that 45 isolates belonged to the genome type D1. Restriction site maps of a prototype D1 and of all deviating restriction variants were elaborated for enzymes BamHI, BglII, Hind-III and SalI.

摘要

对60株8型腺病毒（AV8）分离株（于1961年至1982年期间收集，大多来自西德）的DNA用6种核酸内切酶进行了分析，结果显示有6种不同的基因组类型，这表明AV8的变异性相对较低。发现45株分离株属于基因组类型D1。针对BamHI、BglII、Hind-III和SalI酶，构建了D1原型株和所有偏离的限制性变体的限制性酶切图谱。