关于印度鲤（Labeo rohita）和条纹鳢（Channa striatus）鱼卵的脂质分布及脂肪酸组成的研究。

Studies on lipid profiles and fatty acid composition of roe from rohu (Labeo rohita) and murrel (Channa striatus).

作者信息

Prabhakara Rao P G, Jyothirmayi T, Karuna M S L, Prasad R B N

机构信息

Central Food Technological Research Institute, Resource Centre, Hyderabad, India.

出版信息

J Oleo Sci. 2010;59(10):515-9. doi: 10.5650/jos.59.515.

DOI:10.5650/jos.59.515

PMID:20877143

Abstract

The lipid profiles and fatty acid composition of roes from two common fresh water fish viz., rohu (Labeo rohita) and murrel (Channa striatus) have been studied. The dehydrated fish egg was ground to fine powder and extracted with chloroform/methanol (2:1, v/v) to recover the total lipid in 20.2 and 22.7% on dry weight basis. The fat free meal was found to contain 70 and 58% protein in rohu and murrel, respectively. The total lipids were separated into neutral lipids, glycolipids and phospholipids using silica gel column chromatography and found to be 43.8, 72.9% neutral lipids; 12.7, 9.4% glycolipids and 43.5, 17.7% phospholipids in rohu and murrel roes, respectively. The fatty acid compositions of all lipid classes were analysed by GC and GC-MS. Among the saturated fatty acids, hexadecanoic acid (16:0) was found to be 30.2 and 30.4% respectively in rohu and murrel total lipid. n-3 Fatty acids namely eicosapentaenoic acid (20:5, EPA) was observed to an extent of 1.5 and 0.6% and docosahexaenoic acid (22:6, DHA) to an extent of 11.8 and 6.1% respectively in rohu and murrel total lipids. Stearic acid (18:0) was also seen at 12.2 and 6% respectively in rohu and murrel lipids. The fatty acid composition was found to be almost similar in all the lipid classes. The study indicated that rohu and murrel fish egg lipids are good sources for polyunsaturated n-3 fatty acids.

摘要

对两种常见淡水鱼——印度鲤鱼（Labeo rohita）和条纹鳢（Channa striatus）的鱼卵的脂质概况和脂肪酸组成进行了研究。将脱水鱼卵研磨成细粉，并用氯仿/甲醇（2:1，v/v）萃取，以干重计分别回收了20.2%和22.7%的总脂质。发现印度鲤鱼和条纹鳢的脱脂鱼卵粉分别含有70%和58%的蛋白质。使用硅胶柱色谱法将总脂质分离为中性脂质、糖脂和磷脂，发现印度鲤鱼和条纹鳢鱼卵中的中性脂质分别为43.8%、72.9%；糖脂分别为12.7%、9.4%；磷脂分别为43.5%、17.7%。通过气相色谱（GC）和气相色谱 - 质谱联用（GC-MS）分析了所有脂质类别的脂肪酸组成。在饱和脂肪酸中，十六烷酸（16:0）在印度鲤鱼和条纹鳢的总脂质中分别占30.2%和30.4%。n-3脂肪酸即二十碳五烯酸（20:5，EPA）在印度鲤鱼和条纹鳢的总脂质中的含量分别为1.5%和0.6%，二十二碳六烯酸（22:6，DHA）的含量分别为11.8%和6.1%。硬脂酸（18:0）在印度鲤鱼和条纹鳢的脂质中的含量分别为12.2%和6%。发现所有脂质类别的脂肪酸组成几乎相似。该研究表明，印度鲤鱼和条纹鳢的鱼卵脂质是多不饱和n-3脂肪酸的良好来源。