Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Pediatr Infect Dis J. 2010 Oct;29(10):931-3. doi: 10.1097/inf.0b013e3181e2062e.
Viruses are the most common causes of respiratory tract infections in children. The implementation of rapid virology assays can identify the most common pathogens involved. This study was undertaken on Iranian children less than 6 years old with respiratory infection.
A total of 202 specimens were tested for the presence of 9 respiratory viruses by developing 3 multiplex reverse transcription-polymerase chain reaction and 1 uniplex polymerase chain reaction assays.
Viral pathogens were detected in 92 samples (45.5%) with 5.4% having dual infections. Overall, respiratory syncytial virus was the most frequently identified virus (16.8%), followed by adenovirus (14.4%), influenza A virus (4.9%), parainfluenza virus-3 (4.4%), parainfluenza virus-1 (2.9%), and both influenza B virus and hMPV (0.49).
Respiratory tract infection is a frequent cause of pediatric morbidity and mortality and a common reason for admission in acute care hospitals and outpatients visits. Appropriate diagnostic testing is important for specific diagnosis at an early stage of the illness because of the similarity in clinical presentation of patients with different viral infections caused by several respiratory pathogens.
病毒是儿童呼吸道感染最常见的原因。快速病毒学检测的实施可以鉴定出最常见的病原体。本研究针对伊朗 6 岁以下呼吸道感染的儿童进行。
通过开发 3 种多重逆转录-聚合酶链反应和 1 种单重聚合酶链反应检测，对 202 份标本进行了 9 种呼吸道病毒的检测。
在 92 份样本中检测到了病毒病原体（45.5%），有 5.4%的样本存在双重感染。总的来说，呼吸道合胞病毒是最常被识别的病毒（16.8%），其次是腺病毒（14.4%）、甲型流感病毒（4.9%）、副流感病毒-3（4.4%）、副流感病毒-1（2.9%）以及乙型流感病毒和 hMPV（0.49%）。
呼吸道感染是儿科发病率和死亡率的常见原因，也是急性护理医院和门诊就诊的常见原因。由于几种呼吸道病原体引起的不同病毒感染患者的临床表现相似，因此在疾病早期进行适当的诊断检测对于明确诊断非常重要。
