Tsagarakis Nikolaos J, Sideri Anthi, Makridis Panagiotis, Triantafyllou Argyro, Stamoulakatou Alexandra, Papadogeorgaki Eleni
Central Laboratories, Hygeia General Hospital, Athens, Greece.
Medicine (Baltimore). 2018 Jun;97(22):e10903. doi: 10.1097/MD.0000000000010903.
The FilmArray Respiratory Panel (FA-RP) is an FDA certified multiplex PCR that can detect 17 viruses and 3 bacteria responsible for upper respiratory tract infections, thus it is potentially useful to the assessment of the age-related prevalence of these pathogens.In this observational study, we retrospectively analyzed the results of all the respiratory samples, which had been processed during 1 year-period (November 2015 to November 2016) with the FA-RP, in the Central Laboratories of Hygeia & Mitera General Hospitals of Athens, Greece. In order to have an age-related distribution, the following age groups were implemented: (<2), (≥2, <5), (≥5, <10), (≥10, <18), (≥18, <45), (≥45, <65), and (≥65) years old.Among 656 respiratory samples tested, 362 (55%) were from male and 294 (45%) from female patients, while 356 (54.3%) were positive and 300 (45.7%) negative. In the first age-group (<2), 41/121 samples (33.9%) revealed human rhinovirus/enterovirus (HRV) and 16 (13.2%) adenovirus (Adv), followed by respiratory syncytial virus (RSV), coronavirus, human metapneumovirus (Hmpv), and parainfluenza viruses (PIV). In the age-group (≥2, <5), Adv predominated with 37/147 samples (25.2%), followed by HRV, RSV, coronavirus (all types), and influenza, Hmpv and PIV. In the age-group (≥5, <10), HRV was identified in 25/80 samples (31.3%), Adv in 18 (22.5%), influenza in 11 (13.8%), and Hmpv in 6 (7.5%). Influenza predominated in the age-group (≥10, <18), with 4/22 samples (18.2%), while in the remaining age-groups (≥18), HRV was the commonest isolated pathogen, 33/286 (11.5%), followed by influenza with 20 (7%) (influenza A H1-2009, 11/20).In our patient series, HRV seemed to prevail in most age-groups, followed by Adv, although Influenza was the second most frequent pathogen isolated in the age-groups (≥18). Moreover, increasing age corresponded to increasing possibility of having a negative sample, indicating that FilmArray may be more useful before adolescence.
FilmArray呼吸道检测板（FA-RP）是一种经美国食品药品监督管理局（FDA）认证的多重聚合酶链反应（PCR）检测方法，可检测导致上呼吸道感染的17种病毒和3种细菌，因此对于评估这些病原体与年龄相关的流行情况可能具有重要意义。在这项观察性研究中，我们回顾性分析了希腊雅典Hygeia和Mitera综合医院中央实验室在1年期间（2015年11月至2016年11月）使用FA-RP检测的所有呼吸道样本的结果。为了得出与年龄相关的分布情况，划分了以下年龄组：（<2岁）、（≥2岁，<5岁）、（≥5岁，<10岁）、（≥10岁，<18岁）、（≥18岁，<45岁）、（≥45岁，<65岁）和（≥65岁）。在检测的656份呼吸道样本中，362份（55%）来自男性患者，294份（45%）来自女性患者，其中356份（54.3%）呈阳性，300份（45.7%）呈阴性。在第一个年龄组（<2岁）中，41/121份样本（33.9%）检测出人鼻病毒/肠道病毒（HRV），16份（13.2%）检测出腺病毒（Adv），其次是呼吸道合胞病毒（RSV）、冠状病毒、人偏肺病毒（Hmpv）和副流感病毒（PIV）。在（≥2岁，<5岁）年龄组中，Adv占主导地位，37/147份样本（25.2%）检测出该病毒，其次是HRV、RSV、冠状病毒（所有类型）、流感病毒、Hmpv和PIV。在（≥5岁，<10岁）年龄组中，25/80份样本（31.3%）检测出HRV，18份（22.5%）检测出Adv，11份（13.8%）检测出流感病毒，6份（7.5%）检测出Hmpv。流感病毒在（≥10岁，<18岁）年龄组中占主导地位，4/22份样本（18.2%）检测出该病毒，而在其余年龄组（≥18岁）中，HRV是最常见的分离病原体，33/286份样本（11.5%）检测出该病毒，其次是流感病毒，20份样本（7%）（甲型H1-2009流感病毒，11/20）。在我们的患者系列中，HRV似乎在大多数年龄组中占主导地位，其次是Adv，尽管流感病毒是（≥18岁）年龄组中第二常见的分离病原体。此外，年龄越大，样本呈阴性的可能性越大，这表明FilmArray在青春期前可能更有用。
