伊朗18岁及以下因严重急性呼吸道感染住院儿童中鼻病毒和腺病毒的系统发育特征分析

Phylogenetic characterization of rhinovirus and adenovirus in hospitalized children aged ≤ 18 years with severe acute respiratory infection in Iran.

作者信息

Abbasi Simin, Shafiei-Jandaghi Nazanin Zahra, Shadab Azadeh, Hassani Seyed Abbas, Rahimi Foroushani Abbas, Hosseinkhan Nazanin, Aghamir Fahimeh, Mokhtari-Azad Talat, Yavarian Jila

机构信息

Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Department of Pediatric Intensive Care Unit, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Microbiol. 2023 Feb;15(1):155-162. doi: 10.18502/ijm.v15i1.11932.

DOI:10.18502/ijm.v15i1.11932

PMID:37069918

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10105264/

Abstract

BACKGROUND AND OBJECTIVES

Human rhinoviruses (HRVs) and human adenoviruses (HAdVs) are among the most prevalent viruses in hospitalized patients with severe acute respiratory infection (SARI). This study aimed to evaluate the molecular characterization of HRV and HAdV in hospitalized patients with SARI, who aged ≤ 18 years in Tehran, Iran.

MATERIALS AND METHODS

To detect these two viruses, a conventional nested RT-PCR (Reverse transcription-polymerase chain reaction) assay was performed on 264 throat swabs collected from December 2018 to March 2019. The epidemiological data were analyzed and phylogenetic trees were constructed.

RESULTS

Of 264 cases with SARI, 36 (13.6%) and 28 (10.6%) were positive for HAdV and HRV respectively. Of 21 HRV sequenced samples, HRV-A (42.9%), HRV-B (9.5%) and HRV-C (47.6%) and of 36 HAdV sequenced samples, HAdV-C6 (38.9%), HAdV-B7 (22.2%), HAdV-B3 (11.1%), HAdV-B16 (5.6%), HAdV-C5 (13.9%), HAdV-C57 (5.6%), HAdV-E4 (2.8%); were detected in children with SARI. Some viral genotypes appeared to cause more severe disease, which may lead to hospitalization.

CONCLUSION

Large-scale studies are recommended to investigate the epidemiology and molecular characterizations through surveillance networks to provide useful information on etiology, seasonality, and demographic associations in patients with SARI.

摘要

背景与目的

人鼻病毒（HRV）和人腺病毒（HAdV）是重症急性呼吸道感染（SARI）住院患者中最常见的病毒之一。本研究旨在评估伊朗德黑兰年龄≤18岁的SARI住院患者中HRV和HAdV的分子特征。

材料与方法

为检测这两种病毒，对2018年12月至2019年3月收集的264份咽拭子进行了常规巢式逆转录聚合酶链反应（RT-PCR）检测。分析了流行病学数据并构建了系统发育树。

结果

在264例SARI病例中，HAdV和HRV阳性分别为36例（13.6%）和28例（10.6%）。在21份测序的HRV样本中，HRV-A（42.9%）、HRV-B（9.5%）和HRV-C（47.6%），在36份测序的HAdV样本中，HAdV-C6（38.9%）、HAdV-B7（22.2%）、HAdV-B3（11.1%）、HAdV-B16（5.6%）、HAdV-C5（13.9%）、HAdV-C57（5.6%）、HAdV-E4（2.8%）；在SARI儿童中被检测到。一些病毒基因型似乎会导致更严重的疾病，这可能导致住院。