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当前用于检测微生物病原体的分子和新兴纳米生物技术方法。

Current molecular and emerging nanobiotechnology approaches for the detection of microbial pathogens.

机构信息

Department of Microbiology and Plant Pathology, University of Pretoria, South Africa.

出版信息

Crit Rev Microbiol. 2010 Nov;36(4):318-39. doi: 10.3109/1040841X.2010.489892.

Abstract

Waterborne microbial diseases are escalating worldwide increasing the need for powerful and sensitive diagnostics tools. Molecular methodologies, including immunological and nucleic acid-based methods, have only recently been applied in the water sector. Advances in nanotechnology and nanomaterials have opened the door for the development of new diagnostic tools with increased sensitivity and speed, and reduced cost and labor. Quantum dots, flo dots, gold nanoparticles, magnetic nanoparticles, carbon nanotubes, nanowires, and nanocantilevers, with their unique optical and physical properties, have already been applied in nanodiagnostics. Nanobiotechnology, once remaining technical and practical problems has been addressed, will play an important role in the detection of microbial pathogens.

摘要

水传播微生物疾病在全球范围内不断升级,这增加了对强大而敏感的诊断工具的需求。分子方法,包括免疫学和基于核酸的方法,最近才被应用于水领域。纳米技术和纳米材料的进步为开发具有更高灵敏度和速度、更低成本和劳动力的新型诊断工具开辟了道路。量子点、荧光点、金纳米粒子、磁性纳米粒子、碳纳米管、纳米线和纳米悬臂梁,由于其独特的光学和物理特性,已经被应用于纳米诊断。一旦纳米生物技术解决了仍然存在的技术和实际问题,它将在微生物病原体的检测中发挥重要作用。

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