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支原体感染对基因表达研究的干扰：是环境而非基因。

Interference of Mycoplasma infection in a gene expression study: it was the environment and not the gene.

机构信息

Division of Medical and Chemical Laboratory Diagnosis, Department of Laboratory Medicine, Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.

出版信息

Appl Environ Microbiol. 2010 Dec;76(23):7867-9. doi: 10.1128/AEM.01265-10. Epub 2010 Oct 1.

DOI:10.1128/AEM.01265-10
PMID:20889783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2988611/
Abstract

We show that short-term exposure to doxycycline, as used in tetracycline-inducible gene expression models, protects cells from stress-induced death in cultures infected with Mycoplasma arginini. Coinciding with the expected maximum level of gene activity, antimicrobial effects of tetracyclines might be mistaken for antiapoptotic properties of the expressed gene product.

摘要

我们证明，在感染精氨酸支原体的培养物中，短期接触多西环素（如四环素诱导基因表达模型中所使用的那样）可保护细胞免受应激诱导的死亡。四环素的抗菌作用与预期的最大基因活性水平一致，可能会被误认为是表达基因产物的抗凋亡特性。

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本文引用的文献

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PCR-based detection and eradication of mycoplasmal infections from various mammalian cell lines: a local experience.基于 PCR 的检测和消除各种哺乳动物细胞系中的支原体感染：本地经验。
Cytotechnology. 2009 Dec;61(3):117-24. doi: 10.1007/s10616-010-9252-6. Epub 2010 Feb 6.
2
High-throughput detection and multiplex identification of cell contaminations.细胞污染的高通量检测与多重鉴定
Nucleic Acids Res. 2009 Oct;37(18):e119. doi: 10.1093/nar/gkp581. Epub 2009 Jul 9.
3
Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention.支原体污染细胞培养：发生率、来源、影响、检测、清除、预防。
Cytotechnology. 2002 Jul;39(2):75-90. doi: 10.1023/A:1022913015916.
4
Evaluation of a protocol for molecular broad-range diagnosis of culture-negative bacterial infections in clinical routine diagnosis.临床常规诊断中用于培养阴性细菌感染分子广谱诊断的方案评估。
J Appl Microbiol. 2008 Apr;104(4):1228-37. doi: 10.1111/j.1365-2672.2007.03648.x. Epub 2007 Nov 20.
5
A comparative study examining the cytotoxicity of inducible gene expression system ligands in different cell types.
Toxicol In Vitro. 2008 Feb;22(1):261-6. doi: 10.1016/j.tiv.2007.08.019. Epub 2007 Sep 6.
6
Minocycline inhibits poly(ADP-ribose) polymerase-1 at nanomolar concentrations.米诺环素在纳摩尔浓度下可抑制聚（ADP - 核糖）聚合酶 -1。
Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9685-90. doi: 10.1073/pnas.0600554103. Epub 2006 Jun 12.
7
Schlafen-1 causes a cell cycle arrest by inhibiting induction of cyclin D1.睡眠蛋白-1通过抑制细胞周期蛋白D1的诱导来引起细胞周期停滞。
J Biol Chem. 2005 Sep 2;280(35):30723-34. doi: 10.1074/jbc.M500435200. Epub 2005 Jun 9.
8
Tet B or not tet B: advances in tetracycline-inducible gene expression.有四环素诱导型基因表达系统还是没有：四环素诱导型基因表达的进展
Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):797-9. doi: 10.1073/pnas.96.3.797.
9
Schlafen, a new family of growth regulatory genes that affect thymocyte development.“睡眠”，一个影响胸腺细胞发育的生长调节基因新家族。
Immunity. 1998 Nov;9(5):657-68. doi: 10.1016/s1074-7613(00)80663-9.
10
Serological identification of mycoplasmas by growth and metabolic inhibition tests.
Methods Mol Biol. 1998;104:105-11. doi: 10.1385/0-89603-525-5:105.