人外周血单个核细胞、鲎试剂与家兔用于检测热原质的比较。
Comparison of cultured human mononuclear cells, Limulus amebocyte lysate and rabbits in the detection of pyrogens.
作者信息
Hansen E W, Christensen J D
机构信息
Royal Danish School of Pharmacy, Department of Biological Sciences, Copenhagen.
出版信息
J Clin Pharm Ther. 1990 Dec;15(6):425-33. doi: 10.1111/j.1365-2710.1990.tb00407.x.
Isolated human mononuclear cells exposed to either lipopolysaccharide or Staphylococcus aureus secreted interleukin-1 like material. The secretion was concentration dependent. The minimal detectable level in the test solution of lipopolysaccharide and Staphylococcus aureus was 200 pg/ml and 10(5) cells/ml respectively. The sensitivity and specificity of the Limulus Amebocyte Lysate test, the rabbit pyrogen test and the monocyte test are compared. The monocyte test is proposed as an alternative in-vitro test to the rabbit pyrogen test.
暴露于脂多糖或金黄色葡萄球菌的分离人单核细胞分泌白细胞介素-1样物质。这种分泌呈浓度依赖性。脂多糖和金黄色葡萄球菌测试溶液中的最低可检测水平分别为200 pg/ml和10⁵个细胞/ml。比较了鲎试剂试验、家兔热原试验和单核细胞试验的敏感性和特异性。提出单核细胞试验可作为家兔热原试验的替代体外试验。
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