Meyle Eva, Stroh Petra, Günther Frank, Hoppy-Tichy Torsten, Wagner Christof, Hänsch G Maria
Institute of Immunology, University of Heidelberg, Germany.
Int J Artif Organs. 2010 Sep;33(9):608-20. doi: 10.1177/039139881003300906.
Bacteria organized in biofilms are a common cause of relapsing or persistent infections, and the ultimate cause of implant-associated osteomyelitis. In these patients, biofilms of staphylococci are prevalent. Bacteria organized as biofilms are relatively resistant towards antibiotics and biocides, and it is also assumed that they may escape host defense mechanisms. In this context, we have studied how polymorphonuclear neutrophils (PMN), the "first line of defense" against bacterial infection, interact with biofilms generated in vitro. We found that PMN recognize biofilms and activate defense-associated reactions, including phagocytosis, degranulation of lactoferrin and elastase, and DNA release as well. Destruction of biofilms ensues, showing that biofilms are not inherently protected against the attack by phagocytic cells.
形成生物膜的细菌是复发性或持续性感染的常见原因，也是植入物相关骨髓炎的最终病因。在这些患者中，葡萄球菌生物膜很常见。形成生物膜的细菌对抗生素和杀菌剂具有相对抗性，并且还认为它们可能逃避宿主防御机制。在此背景下，我们研究了作为抵抗细菌感染的“第一道防线”的多形核中性粒细胞（PMN）如何与体外产生的生物膜相互作用。我们发现PMN能够识别生物膜并激活与防御相关的反应，包括吞噬作用、乳铁蛋白和弹性蛋白酶的脱颗粒以及DNA释放。随后生物膜被破坏，这表明生物膜并非天然就能抵御吞噬细胞的攻击。
