Fogh-Andersen N, Thomsen J K, Foldager N, Siggaard-Andersen O
Department of Clinical Chemistry, Herlev Hospital, Denmark.
Scand J Clin Lab Invest Suppl. 1990;203:247-52. doi: 10.3109/00365519009087518.
An easy method to measure blood volume is clinically needed. We used carbon monoxide (CO) and the OSM3 to measure circulating hemoglobin and blood volume with the indicator dilution principle. 50 mL of CO was administered into a closed rebreathing system and taken up via the lungs, and the amount of hemoglobin in the blood was calculated from the increase in carboxyhemoglobin fraction after 10 min. Blood volume was calculated by division with the concentration of hemoglobin. We observed that the absorption spectrum of carboxyhemoglobin (COHb) depends on pH and pCO2, which must be controlled when very accurate spectrophotometry is necessary. The bias is 3% COHb per pH unit during calibration of the OSM3, which may be permissible for patients with CO poisoning, but not for the present purpose. With this in mind the method is very accurate, precise and simple.
临床上需要一种简单的测量血容量的方法。我们使用一氧化碳（CO）和OSM3，根据指示剂稀释原理来测量循环血红蛋白和血容量。将50 mL的CO注入一个封闭的再呼吸系统中，通过肺部摄取，10分钟后根据羧基血红蛋白分数的增加来计算血液中的血红蛋白量。通过血红蛋白浓度相除来计算血容量。我们观察到羧基血红蛋白（COHb）的吸收光谱取决于pH值和二氧化碳分压（pCO2），在需要非常精确的分光光度法时必须对此进行控制。在OSM3校准期间，每pH单位的偏差为3%的COHb，这对于一氧化碳中毒患者可能是允许的，但不符合当前目的。考虑到这一点，该方法非常准确、精确且简单。
