The Centre for Physical Activity Research, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark;
Center for Integrative Human Physiology, Institute of Physiology, University of Zürich, Zürich, Switzerland; and.
J Appl Physiol (1985). 2017 Sep 1;123(3):645-654. doi: 10.1152/japplphysiol.00185.2017. Epub 2017 Jun 29.
In this Cores of Reproducibility in Physiology (CORP) article, we present the theory and practical aspects of the carbon monoxide (CO) rebreathing method for the determination of total hemoglobin mass in humans. With CO rebreathing, a small quantity of CO is diluted in O and rebreathed for a specified time period, during which most of the CO is absorbed and bound to circulating hemoglobin. The dilution principle then allows calculation of the total number of circulating hemoglobin molecules based on the number of absorbed CO molecules and the resulting changes in the fraction of carboxyhemoglobin in blood. Total hemoglobin mass is derived by multiplication with the molar weight of hemoglobin. CO rebreathing has been used for >100 yr and has undergone steady improvement so that today excellent values in terms of accuracy and precision can be achieved if the methodological precautions are carefully followed.
在这篇生理学可重复性核心（CORP）文章中，我们介绍了一氧化碳（CO）重呼吸法测定人体总血红蛋白质量的理论和实际方面。在 CO 重呼吸中，将少量的 CO 稀释在 O 中并在特定时间段内重呼吸，在此期间，大部分 CO 被吸收并与循环中的血红蛋白结合。然后，稀释原理允许根据吸收的 CO 分子数量以及血液中碳氧血红蛋白的分数变化来计算循环血红蛋白分子的总数。总血红蛋白质量通过与血红蛋白的摩尔质量相乘来推导。CO 重呼吸已经使用了 100 多年，并不断得到改进，因此，如果仔细遵循方法学注意事项，今天可以获得出色的准确性和精密度值。
