Weislow O S, Allen P T, Shepherd R E, Twardzik D R, Fowler A K, Hellman A
J Natl Cancer Inst. 1978 Jul;61(1):123-9. doi: 10.1093/jnci/61.1.123.
A single ip inoculation of female, outbred Sprague-Dawley rats with a viable mouse xenotropic type C virus significantly reduced the incidence and/or retarded the development of mammary carcinoma induced by 7, 12-dimethylbenz[a]anthracene administered orally 7 days after virus. Although infectious virus could not be isolated from organs of infected rats, high titers of circulating and tumor-associated antibodies were detected against the viral internal core protein p30, and a low-grade antibody response to intact virus or envelope glycoprotein was found. Moreover, a cell-mediated immune response, measured by lymphocyte transformation, was detected with the use of intact virus but not with p30 antigen. No immunity developed after a single inoculation of UV-inactivated virus. These data indicated that inoculation of adult individuals of heterologous species with viable xenotropic mouse type C virus resulted in the rapid disappearance of infectious virus from the recipient, followed by the development of both humoral and cellular immunity to virion constituents. These events led, by unknown mechanisms, to the effective retardation of chemical carcinogenesis when infection preceded carcinogen administration.
用活的小鼠嗜异性C型病毒对雌性远交群Sprague-Dawley大鼠进行单次腹腔注射，可显著降低在病毒接种7天后口服7,12-二甲基苯并[a]蒽诱导的乳腺癌的发病率和/或延缓其发展。尽管从感染大鼠的器官中无法分离出感染性病毒，但检测到针对病毒内部核心蛋白p30的高滴度循环抗体和肿瘤相关抗体，并且发现对完整病毒或包膜糖蛋白有低度抗体反应。此外，通过淋巴细胞转化测量的细胞介导免疫反应，在用完整病毒时可检测到，而用p30抗原时则检测不到。单次接种紫外线灭活病毒后未产生免疫力。这些数据表明，用活的嗜异性小鼠C型病毒接种异种成年个体，导致受体中感染性病毒迅速消失，随后对病毒粒子成分产生体液免疫和细胞免疫。当感染先于致癌物给药时，这些事件通过未知机制有效地延缓了化学致癌作用。
