Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, 268 Grosvenor Street, London, ON N6A 4V2, Canada.
Can J Microbiol. 2010 Sep;56(9):730-8. doi: 10.1139/w10-061.
Microbial exposure may direct the immune system away from allergic-type responses, but until now probiotic interventions have had limited success in the prevention and treatment of allergic diseases. In this study, a novel probiotic mixture was specifically created based on preliminary in vitro investigations on pollen-induced immune responses. A mixture with Lactobacillus rhamnosus GR-1 and a novel fecal Bifidobacterium adolescentis isolate was formulated into a yogurt and tested for its effects in 36 subjects with allergic rhinitis over 2 pollen seasons in a double-blind, placebo-controlled trial. The new formulation was well tolerated, but did not have significant effects on the quality of life scores, use of antihistamines, or eosinophil cationic protein concentration in nasal lavage. However, at the end of the grass pollen season, serum IL-10 and IL-12 levels were increased in the probiotic group compared to the controls. During the ragweed season, the serum TGF-β levels were significantly higher in the probiotic group than in the controls. In conclusion, the novel probiotic formulation had potentially desirable effects on the cytokine profile of patients with allergic rhinitis, but provided few clinical benefits. The study highlights the challenges in designing efficient immunomodulatory probiotic therapies based upon in vitro findings.
微生物暴露可能使免疫系统远离过敏反应类型的反应，但到目前为止，益生菌干预措施在预防和治疗过敏性疾病方面收效甚微。在这项研究中，根据花粉诱导的免疫反应的初步体外研究，专门创建了一种新型益生菌混合物。将鼠李糖乳杆菌 GR-1 和一种新型粪便双歧杆菌分离株的混合物制成酸奶，并在一项双盲、安慰剂对照试验中在 36 名过敏性鼻炎患者中测试了两个花粉季节的效果。新配方耐受性良好，但对生活质量评分、抗组胺药的使用或鼻灌洗液中嗜酸性粒细胞阳离子蛋白浓度没有显著影响。然而，在草花粉季节结束时，与对照组相比，益生菌组的血清 IL-10 和 IL-12 水平升高。在豚草花粉季节，益生菌组的血清 TGF-β 水平明显高于对照组。总之，新型益生菌配方对过敏性鼻炎患者的细胞因子谱可能有理想的影响，但提供的临床益处很少。该研究强调了基于体外发现设计有效免疫调节益生菌疗法的挑战。
