Zhernosekova I V, Kukushkina N V, Babenko Iu S
Antibiot Khimioter. 1990 Nov;35(11):10-3.
The combined effect of ethonium, a synthetic surface active substance and preparations of lytic enzymes on staphylococcal cells and possible use of ethonium for preprocessing the staphylococcal biomass were studied. With addition of ethonium to the reaction system the activity of the enzyme preparations against both the intact staphylococcal cells and the preheated ones markedly increased. The maximum activity of the lytic enzymes was observed when the substance concentration was equal to 25 mg/l. Preprocessing of the biomass with 0.05% ethonium solution increased the rate and strength of the enzymatic lysis. The data are indicative of possible decrease of the concentration of the lytic enzymes and efficient usage of low active preparations in disintegration of staphylococcal cells in the presence of ethonium.
研究了合成表面活性物质乙铵和溶菌酶制剂对葡萄球菌细胞的联合作用，以及乙铵用于预处理葡萄球菌生物质的可能性。向反应体系中添加乙铵后，酶制剂对完整葡萄球菌细胞和预热葡萄球菌细胞的活性均显著提高。当物质浓度等于25mg/L时，观察到溶菌酶的最大活性。用0.05%乙铵溶液预处理生物质可提高酶解的速率和强度。这些数据表明，在乙铵存在的情况下，溶菌酶的浓度可能降低，并且低活性制剂可有效地用于葡萄球菌细胞的裂解。
