Department of Pharmacology, Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, United States.
Curr Opin Genet Dev. 2010 Dec;20(6):581-7. doi: 10.1016/j.gde.2010.08.009.
The circadian clock is an endogenous oscillator that regulates daily rhythms in behavior and physiology. In recent years, systems biology and genomics approaches re-shaped our view of the clock. Our understanding of outputs that regulate behavior and physiology has been enhanced through gene expression profiling and proteomic analyses. Systems approaches uncovered underlying principles of transcriptional regulation and robustness of the oscillator through perturbation analysis and synthetic methods. Finally, new clock components and modifiers were identified through cell-based screening efforts and proteomics.
生物钟是一种内源性振荡器，调节行为和生理的日常节律。近年来，系统生物学和基因组学方法改变了我们对时钟的看法。通过基因表达谱和蛋白质组学分析，我们对调节行为和生理的输出有了更深入的了解。系统方法通过扰动分析和合成方法揭示了转录调控的基本原理和振荡器的稳健性。最后，通过基于细胞的筛选工作和蛋白质组学鉴定了新的时钟组件和调节剂。
