School of Pharmacy and Medical Sciences, The University of South Australia, North Terrace, Adelaide, Australia.
J Chem Ecol. 2010 Nov;36(11):1171-9. doi: 10.1007/s10886-010-9869-0. Epub 2010 Oct 7.
Results of experiments conducted on ink recovered from the squid Sepioteuthis australis indicate that there is no epinephrine or protein naturally present in the ink as it would be ejected in vivo. Protein content was effectively zero when ink was syringed from the duct end of the ink sac of freshly killed animals. By contrast, there were proteins in samples collected from dead specimens where ink was collected by a stripping method. From these samples, a single large molecular weight protein was identified as having tyrosinase activity. Digestion of syringed ink did not yield signs of melanin-bound proteins. Analysis of supernatants after centrifugation of squid ink consistently revealed the presence of DOPA, dopamine, and taurine, whereas epinephrine and nor-epinephrine were recorded from what was believed to be contaminated ink. Histological investigations of the ink sac revealed a compartmentalised glandular structure distal to the duct end. Closer observation of the glandular tissue showed that compartments increased in size as they matured and moved further into the lumen. It was concluded that the presence of epinephrine and tyrosinase (or a related protein) in the ink of S. australis could be attributed to rupturing of basal glandular compartments or contamination from other sources during the extraction process.
从鱿鱼（Sepioteuthis australis）中回收的墨水中的实验结果表明，作为活体喷射的墨水中，没有肾上腺素或蛋白质。从刚死亡的动物的墨囊导管端用注射器抽取的墨水中，蛋白质含量实际上为零。相比之下，在使用剥离法从死亡标本中收集墨汁的样本中，存在具有酪氨酸酶活性的单一高分子量蛋白质。注射器抽取的墨汁消化后，没有发现与黑色素结合的蛋白质的迹象。离心鱿鱼墨汁后的上清液分析一致显示存在 DOPA、多巴胺和牛磺酸，而肾上腺素和去甲肾上腺素则来自于被认为是受污染的墨汁。墨囊的组织学研究揭示了导管末端远处存在一个分隔的腺状结构。对腺组织的更仔细观察表明，随着腺腔的成熟和进一步进入管腔，腔室的大小会增加。因此得出结论，在 S. australis 的墨水中存在肾上腺素和酪氨酸酶（或相关蛋白），这可能归因于在提取过程中基础腺腔室破裂或来自其他来源的污染。
