Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Langmuir. 2010 Nov 16;26(22):17520-7. doi: 10.1021/la1029728. Epub 2010 Oct 7.
Thiolated dextran-coated gold nanorods (DEX-GNRs) were synthesized for targeted delivery to inflammatory macrophages and their photothermal ablation under near-infrared (NIR) light irradiation. Successful synthesis of DEX-GNRs was achieved using thiolated dextran, generated by applying mercaptopropionic acid to transform a hydroxyl group of dextran into a thiol group which has strong binding affinity with surfaces of GNRs. We confirmed both the existence of a thiol group in the functionalized dextran using Ellman's reagent in a thiol group assay and the characteristic band of DEX-GNRs using FT-IR spectrum. Furthermore, a cellular uptake study revealed that dextran showed a superior ability to bind the GNRs surface against macrophages compared to those of PEGylated GNRs with various molecular weights of polyethyleneglycol (PEG). Consequently, an in vitro photothermal irradiation experiment using NIR light indicated that DEX-GNRs exhibited a significant cell-killing efficacy, even with a lower concentration of Au and a low-power light source.
巯基化葡聚糖修饰的金纳米棒（DEX-GNRs）被合成用于靶向递送至炎症巨噬细胞，并在近红外（NIR）光照射下进行光热消融。通过应用巯基丙酸将葡聚糖上的一个羟基转化为巯基，从而生成具有与 GNRs 表面强结合亲和力的巯基化葡聚糖，成功合成了 DEX-GNRs。我们使用巯基组测定法中的 Ellman 试剂证实了功能化葡聚糖中存在巯基，并且使用傅里叶变换红外（FT-IR）光谱证实了 DEX-GNRs 的特征带。此外，细胞摄取研究表明，与具有不同分子量的聚乙二醇（PEG）的 PEGylated GNRs 相比，葡聚糖具有与 GNRs 表面结合的优越能力。因此，使用 NIR 光进行的体外光热照射实验表明，DEX-GNRs 即使在较低浓度的 Au 和低功率光源下，也表现出显著的细胞杀伤效果。
