Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
Semin Cancer Biol. 2010 Oct;20(5):304-11. doi: 10.1016/j.semcancer.2010.10.003. Epub 2010 Oct 8.
This article considers the fidelity of DNA replication performed by eukaryotic DNA polymerases involved in replicating the nuclear genome. DNA replication fidelity can vary widely depending on the DNA polymerase, the composition of the error, the flanking sequence, the presence of DNA damage and the ability to correct errors. As a consequence, defects in processes that determine DNA replication fidelity can confer strong mutator phenotypes whose specificity can help determine the molecular nature of the defect.
本文考虑了真核生物参与核基因组复制的 DNA 聚合酶所进行的 DNA 复制保真度。DNA 复制保真度可能因 DNA 聚合酶、错误的组成、侧翼序列、DNA 损伤的存在以及纠错能力的不同而有很大差异。因此，决定 DNA 复制保真度的过程缺陷可能赋予强烈的突变速率表型，其特异性有助于确定缺陷的分子性质。
