The University of Queensland, Institute for Molecular Bioscience, Department of Molecular Cell Biology, 4072 - St Lucia, Brisbane, QLD, Australia.
Curr Mol Med. 2010 Nov;10(8):763-73. doi: 10.2174/156652410793384204.
The molecular mechanism of membrane fusion essential to vital cellular activities such as intracellular transport, hormone secretion, enzyme release, or neurotransmission, involve the assembly and disassembly of a specialized set of proteins in opposing bilayers. Recent evidences shed new light on the role Ca(2+) has in the regulation of this mechanism in which the Golgi apparatus works as a central station; from here, Ca(2+) ions are released into and recovered from the cytosol during the different steps of the cargo progression. In fact, transient cytosolic Ca(2+) fluctuations take a crucial role to recruit proteins and enzymes Ca(2+)-sensitive on Golgi membranes where they are involved in membranes remodelling which is fundamental process for the fusion events that allow protein trafficking. Here I provide an overview of the role Ca(2+) plays in intra-Golgi trafficking underlying some interesting aspects to clarify the mechanisms of cargo progression.
参与细胞内运输、激素分泌、酶释放或神经递质传递等重要细胞活动的膜融合的分子机制，涉及到在相对的双层膜中一组特殊蛋白质的组装和拆卸。最近的证据揭示了 Ca(2+) 在调节该机制中的作用，其中高尔基体作为中央站；在这里，Ca(2+)离子在货物运输的不同步骤中被释放到细胞质中并从中回收。事实上，短暂的细胞质 Ca(2+)波动在招募高尔基体膜上的 Ca(2+)-敏感蛋白和酶方面起着至关重要的作用，它们参与了膜重塑，这是融合事件的基本过程，融合事件允许蛋白质运输。在这里，我概述了 Ca(2+)在高尔基体内部运输中的作用，强调了一些有趣的方面，以阐明货物运输的机制。
