Division of Molecular Cell Biology, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, 4072 Brisbane, St. Lucia, QLD, Australia.
Adv Exp Med Biol. 2012;740:439-60. doi: 10.1007/978-94-007-2888-2_18.
As with other complex cellular functions, intracellular membrane transport involves the coordinated engagement of a series of organelles and machineries; in the last couple of decades more importance has been given to the role of calcium (Ca(2+)) in the regulation of membrane trafficking, which is directly involved in coordinating the endoplasmic reticulum-to-Golgi-to-plasma membrane delivery of cargo. Consequently, the Golgi apparatus (GA) is now considered not just the place proteins mature in as they move to their final destination(s), but it is increasingly viewed as an intracellular Ca(2+) store. In the last few years the mechanisms regulating the homeostasis of Ca(2+) in the GA and its role in membrane trafficking have begun to be elucidated. Here, these recent discoveries that shed light on the role Ca(2+) plays as of trigger of different steps during membrane trafficking has been reviewed. This includes recruitment of proteins and SNARE cofactors to the Golgi membranes, which are both fundamental for the membrane remodeling and the regulation of fusion/fission events occurring during the passage of cargo across the GA. I conclude by focusing attention on Ca(2+) homeostasis dysfunctions in the GA and their related pathological implications.
与其他复杂的细胞功能一样，细胞内膜运输涉及一系列细胞器和机器的协调参与；在过去的几十年中，钙 (Ca(2+)) 在调节膜运输中的作用受到了更多的重视，膜运输直接参与协调内质网-高尔基体-质膜货物的传递。因此，高尔基体（GA）现在不仅被认为是蛋白质在向最终目的地移动时成熟的地方，而且越来越被视为细胞内 Ca(2+) 储存库。在过去的几年中，调节 GA 中 Ca(2+) 动态平衡及其在膜运输中的作用的机制开始被阐明。在这里，综述了最近的发现，这些发现阐明了 Ca(2+) 在膜运输不同步骤中作为触发物的作用。这包括招募蛋白质和 SNARE 辅助因子到高尔基体膜，这对于膜重塑以及调节货物穿过 GA 时发生的融合/裂变事件都是至关重要的。最后，我将重点关注 GA 中 Ca(2+) 动态平衡失调及其相关的病理意义。
