Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4592-8. doi: 10.1073/pnas.1011383107. Epub 2010 Oct 11.
Periodontal disease has been associated with atherosclerosis, suggesting that bacteria from the oral cavity may contribute to the development of atherosclerosis and cardiovascular disease. Furthermore, the gut microbiota may affect obesity, which is associated with atherosclerosis. Using qPCR, we show that bacterial DNA was present in the atherosclerotic plaque and that the amount of DNA correlated with the amount of leukocytes in the atherosclerotic plaque. To investigate the microbial composition of atherosclerotic plaques and test the hypothesis that the oral or gut microbiota may contribute to atherosclerosis in humans, we used 454 pyrosequencing of 16S rRNA genes to survey the bacterial diversity of atherosclerotic plaque, oral, and gut samples of 15 patients with atherosclerosis, and oral and gut samples of healthy controls. We identified Chryseomonas in all atherosclerotic plaque samples, and Veillonella and Streptococcus in the majority. Interestingly, the combined abundances of Veillonella and Streptococcus in atherosclerotic plaques correlated with their abundance in the oral cavity. Moreover, several additional bacterial phylotypes were common to the atherosclerotic plaque and oral or gut samples within the same individual. Interestingly, several bacterial taxa in the oral cavity and the gut correlated with plasma cholesterol levels. Taken together, our findings suggest that bacteria from the oral cavity, and perhaps even the gut, may correlate with disease markers of atherosclerosis.
牙周病与动脉粥样硬化有关，这表明口腔中的细菌可能有助于动脉粥样硬化和心血管疾病的发展。此外，肠道微生物群可能会影响与动脉粥样硬化相关的肥胖。使用 qPCR，我们发现细菌 DNA 存在于动脉粥样硬化斑块中，并且 DNA 的数量与动脉粥样硬化斑块中的白细胞数量相关。为了研究动脉粥样硬化斑块的微生物组成，并检验口腔或肠道微生物群可能导致人类动脉粥样硬化的假设，我们使用 454 焦磷酸测序技术对 16S rRNA 基因进行了测序，以调查 15 名动脉粥样硬化患者的动脉粥样硬化斑块、口腔和肠道样本以及健康对照组的口腔和肠道样本中的细菌多样性。我们在所有动脉粥样硬化斑块样本中均鉴定出 Chryseomonas，在大多数样本中鉴定出 Veillonella 和 Streptococcus。有趣的是，动脉粥样硬化斑块中 Veillonella 和 Streptococcus 的组合丰度与它们在口腔中的丰度相关。此外，在同一个体的动脉粥样硬化斑块与口腔或肠道样本中存在几种其他细菌分类群。有趣的是，口腔和肠道中的几种细菌分类群与血浆胆固醇水平相关。总之，我们的研究结果表明，口腔中的细菌，甚至肠道中的细菌，可能与动脉粥样硬化的疾病标志物相关。
