Dal Maschio Marco, Difato Francesco, Beltramo Riccardo, Blau Axel, Benfenati Fabio, Fellin Tommaso
Dept. of Neuroscience and Brain Technologies, Italian Institute of Technology, Via Morego 30, 16163 Genova, Italy.
Opt Express. 2010 Aug 30;18(18):18720-31. doi: 10.1364/OE.18.018720.
Holographic microscopy is increasingly recognized as a promising tool for the study of the central nervous system. Here we present a "holographic module", a simple optical path that can be combined with commercial scanheads for simultaneous imaging and uncaging with structured two-photon light. The present microscope is coupled to two independently tunable lasers and has two principal configurations: holographic imaging combined with galvo-steered uncaging and holographic uncaging combined with conventional scanning imaging. We applied this flexible system for simultaneous two-photon imaging and photostimulation of neuronal cells with complex light patterns, opening new perspectives for the study of brain function in situ and in vivo.
全息显微镜越来越被认为是研究中枢神经系统的一种有前途的工具。在这里，我们展示了一个“全息模块”，这是一种简单的光路，可以与商业扫描头相结合，用于同时进行结构化双光子光成像和解笼。目前的显微镜与两台独立可调谐激光器耦合，有两种主要配置：全息成像与振镜控制解笼相结合，以及全息解笼与传统扫描成像相结合。我们将这个灵活的系统应用于用复杂光模式对神经元细胞进行同时双光子成像和光刺激，为原位和体内脑功能研究开辟了新的前景。
