Nazirizadeh Yousef, Bog Uwe, Sekula Sylwia, Mappes Timo, Lemmer Uli, Gerken Martina
Institute of Electrical and Information Engineering, Christian-Albrechts-Universität zu Kiel, D-24143 Kiel, Germany.
Opt Express. 2010 Aug 30;18(18):19120-8. doi: 10.1364/OE.18.019120.
There is a strong need for low-cost biosensors to enable rapid, on-site analysis of biological, biomedical, or chemical substances. We propose a platform for label-free optical biosensors based on applying the analyte onto a surface-functionalized photonic crystal slab and performing a transmission measurement with two crossed polarization filters. This dark-field approach allows for efficient background suppression as only the photonic crystal guided-mode resonances interacting with the functionalized surface experience significant polarization rotation. We present a compact biosensor demonstrator using a low-cost light emitting diode and a simple photodiode capable of detecting the binding kinetics of a 2.5 nM solution of the protein streptavidin on a biotin-functionalized photonic crystal surface.
迫切需要低成本的生物传感器，以实现对生物、生物医学或化学物质的快速现场分析。我们提出了一种无标记光学生物传感器平台，该平台基于将分析物应用于表面功能化的光子晶体平板，并使用两个交叉偏振滤光片进行透射测量。这种暗场方法能够有效抑制背景，因为只有与功能化表面相互作用的光子晶体导模共振会经历显著的偏振旋转。我们展示了一种紧凑的生物传感器演示器，它使用低成本发光二极管和简单光电二极管，能够检测2.5 nM链霉亲和素蛋白溶液在生物素功能化光子晶体表面的结合动力学。
