Armed Forces DNA Identification Laboratory, Research Section, 1413 Research Blvd., Suite 101, Rockville, MD 20850, USA.
Forensic Sci Int Genet. 2011 Nov;5(5):415-21. doi: 10.1016/j.fsigen.2010.08.019. Epub 2010 Oct 13.
This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Approximately 1360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples.
本研究开发并鉴定了两个 X 染色体短串联重复序列（STR）多重扩增体系，利用小片段扩增子（小于 200 个碱基对）进行身份鉴定和亲属关系检测，即使是降解的 DNA 也能进行检测。大约 1360 个来自美国 4 个人群群体的样本被用于 15 个 X 染色体 STR 标记的分型：DXS6789、DXS7130、DXS9902、GATA31E08、DXS7424、GATA165B12、DXS101、DXS6795、GATA172D05、DXS10147、DXS8378、DXS7132、DXS6803、HPRTB 和 DXS7423。每个标记都观察到高度多态性，两个多重扩增体系对 200pg 原始 DNA 均具有较高的灵敏度。这两个建议的多重扩增体系适用于法医学应用，并显示出对受损骨骼样本进行分析的潜力。
