Laboratory of Prion Biology, Neurobiology Sector, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Ed. Q1, Basovizza Campus, S.S.14 Km. 163,5, 34149, Trieste, Italy.
Nanoscale. 2010 Dec;2(12):2724-32. doi: 10.1039/c0nr00551g. Epub 2010 Oct 14.
Gold nanoparticles coated with oppositely charged polyelectrolytes, such as polyallylamine hydrochloride and polystyrenesulfonate, were examined for potential inhibition of prion protein aggregation and prion (PrPSc) conversion and replication. Different coatings, finishing with a positive or negative layer, were tested, and different numbers of layers were investigated for their ability to interact and reduce the accumulation of PrPSc in scrapie prion infected ScGT1 and ScN2a cells. The particles efficiently hampered the accumulation of PrPSc in ScN2a cells and showed curing effects on ScGT1 cells with a nanoparticle concentration in the picomolar range. Finally, incubation periods of prion-infected mice treated with nanomolar concentrations of gold nanoparticles were significantly longer compared to untreated controls.
用带相反电荷的聚电解质（如盐酸多聚赖氨酸和聚苯乙烯磺酸钠）包覆的金纳米颗粒，被研究其是否可能抑制朊病毒蛋白聚集和朊病毒（PrPSc）转化和复制。用不同的涂层进行测试，完成带有正层或负层，研究不同数量的层的能力，以相互作用并减少朊病毒感染的 ScGT1 和 ScN2a 细胞中 PrPSc 的积累。这些颗粒有效地阻止了 ScN2a 细胞中 PrPSc 的积累，并对 ScGT1 细胞表现出治疗效果，纳米颗粒浓度在皮摩尔范围内。最后，用纳米颗粒浓度处理的感染朊病毒的小鼠的潜伏期与未处理的对照相比显著延长。
