2000年至2008年期间在斯洛文尼亚从鸽子中分离出的1型禽副粘病毒（新城疫）病毒的分子特征

Molecular characterization of avian paramyxovirus type 1 (Newcastle disease) viruses isolated from pigeons between 2000 and 2008 in Slovenia.

作者信息

Krapez Uros, Steyer Adela Fratnik, Slavec Brigita, Barlic-Maganja Darja, Dovc Alenka, Racnik Jozko, Rojs Olga Zorman

机构信息

Institute of Poultry Health, Veterinary Faculty, University of Ljubljana, Gerbiceva 60, SI-1115 Ljubljana, Slovenia.

出版信息

Avian Dis. 2010 Sep;54(3):1075-80. doi: 10.1637/9161-111709-ResNote.1.

DOI:10.1637/9161-111709-ResNote.1

PMID:20945791

Abstract

Fourteen avian paramyxovirus type 1 (APMV-1; Newcastle disease) viruses isolated from dead free-living and domestic pigeons in Slovenia between 2000 and 2008 were analyzed by a molecular characterization of a part of the fusion protein gene, which included the region encoding the fusion protein cleavage site. Phylogenetic analysis indicated that the Slovene pigeon paramyxovirus type 1 (PPMV-1) viruses do not cluster together but instead are divided into two groups--4bi and 4bii--of sublineage 4b. Nine Slovenian strains were placed in group 4bii. Five other strains clustered together with PPMV-1 from group 4bi. The sequence of the fusion protein cleavage site of all Slovenian strains was typical for pathogenic APMV-1. The 112RRQKRF117 motif was present in the strains from group 4bii, whereas strains from group 4bi displayed the 112GRQKRF117 motif.

摘要

对2000年至2008年间从斯洛文尼亚自由生活和家养的死亡鸽子中分离出的14株1型禽副粘病毒（APMV-1；新城疫）进行了分析，方法是对融合蛋白基因的一部分进行分子特征分析，其中包括编码融合蛋白裂解位点的区域。系统发育分析表明，斯洛文尼亚1型鸽副粘病毒（PPMV-1）病毒并未聚集在一起，而是分为4b亚系的两组——4bi和4bii。9株斯洛文尼亚毒株属于4bii组。其他5株毒株与4bi组的PPMV-1聚集在一起。所有斯洛文尼亚毒株的融合蛋白裂解位点序列都是致病性APMV-1的典型序列。4bii组毒株存在112RRQKRF117基序，而4bi组毒株则显示112GRQKRF117基序。