Reissner J, Herrmann W
Zentrallabor, Bezirkskrankenhauses Meiningen.
Z Med Lab Diagn. 1990;31(8):426-31.
The present paper describes the sensitivity to quantification of changes of HDL-cholesterol in serum by two different precipitation and analytical techniques during the treatment of patients. After the precipitation of VLDL and LDL by phosphotungstic acid/magnesium chloride the chemical determination of HDL-cholesterol in serum with the Liebermann-Burchard reaction yields different results in comparison to enzymatic HDL-cholesterol determined in serum supernatant after the precipitation by polyethylene glycol 20.000. Correlation analyses of apolipoprotein A-I with enzymatic HDL-, HDL2-, HDL3-cholesterol or electrophoretic alpha-cholesterol demonstrate that the therapeutically induced changes (by training and diet) of lipid composition are more correctly reflected by the enzymatic determination of HDL-cholesterol after serum precipitation by polyethylene glycol.
本文描述了在患者治疗过程中，通过两种不同的沉淀和分析技术对血清中高密度脂蛋白胆固醇（HDL-胆固醇）变化进行定量分析的敏感性。用磷钨酸/氯化镁沉淀极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）后，用利伯曼-布赫ard反应对血清中的HDL-胆固醇进行化学测定，与用聚乙二醇20000沉淀后血清上清液中酶法测定的HDL-胆固醇相比，结果不同。载脂蛋白A-I与酶法测定的HDL-胆固醇、HDL2-胆固醇、HDL3-胆固醇或电泳α-胆固醇的相关性分析表明，通过聚乙二醇沉淀血清后酶法测定HDL-胆固醇能更准确地反映（通过训练和饮食）治疗引起的脂质成分变化。
