SahBandar Ivo N, Takahashi Kiyomi, Motomura Kazushi, Djoerban Zubairi, Firmansyah Iman, Kitamura Katsuhiko, Sato Hironori, Pohan Herdiman T, Sato Shigehiro
Department of Microbiology, Iwate Medical University, Morioka, Japan.
AIDS Res Hum Retroviruses. 2011 Jan;27(1):97-102. doi: 10.1089/aid.2010.0163. Epub 2010 Oct 19.
Cocirculation of subtype B and CRF01_AE in Southeast Asia has led to the establishment of new recombinant forms. In our previous study, we found five samples suspected of being recombinants between subtype B and CRF01_AE, and here, we analyzed near full-length sequences of two samples and compared them to known CRFs_01B, subtype B, and CRF01_AE. Five overlapped segments were amplified with nested PCR from PBMC DNA, sequenced, and analyzed for genome mosaicism. The two Indonesian samples, 07IDJKT189 and 07IDJKT194, showed genome-mosaic patterns similar to CRF33_01B references from Malaysia, with one short segment in the 3' end of the p31 integrase-coding region, which was rather more similar to subtype B than CRF01_AE, consisting of unclassified sequences. These results suggest gene-specific continuous diversification and spread of the CRF33_01B genomes in Southeast Asia.
B亚型和CRF01_AE亚型在东南亚的共同传播导致了新重组形式的出现。在我们之前的研究中，我们发现了5个疑似B亚型和CRF01_AE亚型之间重组体的样本，在此，我们分析了其中2个样本的近全长序列，并将它们与已知的CRFs_01B、B亚型和CRF01_AE进行比较。用巢式PCR从外周血单个核细胞（PBMC）DNA中扩增出5个重叠片段，进行测序，并分析基因组镶嵌性。两个印度尼西亚样本07IDJKT189和07IDJKT194显示出与来自马来西亚的CRF33_01B参考序列相似的基因组镶嵌模式，在p31整合酶编码区的3'端有一个短片段，该片段与B亚型的相似性高于CRF01_AE亚型，由未分类序列组成。这些结果表明CRF33_01B基因组在东南亚存在基因特异性的持续多样化和传播。
