Zhang Weiqing, Han Xiaoxu, An Minghui, Zhao Bin, Hu Qinghai, Chu Zhenxing, Xu Jiancheng, Cai Weiping, Chen Xi, Fu Jihua, Wang Zhe, Wu Jianjun, Lu Lin, Zhuang Minghua, Wu Hao, Yan Hongjing, Liao Christina, Takebe Yutaka, Shang Hong
Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
Infectious Disease Department, Guangzhou No. 8 Renmin Hospital, Guangzhou, China.
PLoS One. 2014 Jun 30;9(6):e99693. doi: 10.1371/journal.pone.0099693. eCollection 2014.
The HIV-1 epidemic among men-who-have-sex-with-men (MSM) continues to expand in China. A large-scale national survey we conducted on HIV-1 strains among MSM in 11 provinces in China from 2008 to 2013 (n = 920) identified a novel transmission cluster consisting of six strains (0.7%) that belonged to a new circulating recombinant form (designated CRF59_01B). CRF59_01B contains two subtype B segments of U.S.-European origin (in the pol and vpu-env regions) in a CRF01_AE backbone. CRF59_01B is the second CRF (after CRF55_01B) circulating primarily among MSM in China. CRF59_01B occurs at a low frequency (less than 1%), but it was detected in four different provinces/regions in China: Liaoning (northeast China) (n = 3); Hunan (central China) (n = 1); Guangdong (south China) (n = 1); Yunnan (southwest China) (n = 1). One additional recombinant strain was detected in a heterosexual individual in Liaoning province but is not the focus of this paper. Bayesian molecular clock analyses indicate that CRF59_01B emerged as a result of recombination between CRF01_AE and subtype B around the year 2001. The emergence of multiple forms of recombinants and CRFs reflects the ever-increasing contribution of homosexual transmission in China's HIV epidemic and indicates an active HIV transmission network among MSM in China.
男男性行为者（MSM）中的HIV-1疫情在中国持续蔓延。我们于2008年至2013年在中国11个省份对MSM中的HIV-1毒株进行了大规模全国性调查（n = 920），发现了一个由6株毒株（0.7%）组成的新型传播簇，这些毒株属于一种新的循环重组形式（命名为CRF59_01B）。CRF59_01B在CRF01_AE主干中包含两个源自美国-欧洲的B亚型片段（在pol和vpu-env区域）。CRF59_01B是中国主要在MSM中传播的第二种CRF（仅次于CRF55_01B）。CRF59_01B出现频率较低（低于1%），但在中国四个不同省份/地区被检测到：辽宁（中国东北）（n = 3）；湖南（中国中部）（n = 1）；广东（中国南部）（n = 1）；云南（中国西南部）（n = 1）。在辽宁省一名异性恋个体中检测到另外一株重组毒株，但不是本文的重点。贝叶斯分子钟分析表明，CRF59_01B是2001年左右CRF01_AE与B亚型之间重组的结果。多种重组形式和CRF的出现反映了同性恋传播在中国HIV疫情中日益增加的作用，并表明中国MSM中存在活跃的HIV传播网络。
