Dept of Pathology, Medical College of Wisconsin, 9200 W Wisconsin Ave, Milwaukee, WI 53226, USA.
Am J Clin Pathol. 2010 Nov;134(5):749-61. doi: 10.1309/AJCPFNF5MN1GDWKU.
Data regarding flow cytometry (FC) in nonacute myeloid disorders is confounded by variable gating strategies and controls limited to normal bone marrow (BM) samples. Blasts in diagnostic BM samples of myelodysplastic syndromes (MDSs), myeloproliferative neoplasms (MPNs), and chronic myelomonocytic leukemias (CMMLs) were compared with 20 nonneoplastic cytopenias/cytoses (CCs) and negative staging BM samples using 4-color FC. Blasts in 10 of 20 CCs showed immunophenotypic differences vs control samples. Immunophenotypic alterations were identified in 18 of 21 MDSs, 11 of 14 MPNs, and 7 of 7 CMMLs vs control samples and 13 (62%) of 21 MDSs, 7 (50%) of 14 MPNs, and 3 (43%) of 7 CMMLs vs CCs. Neoplastic-specific blast immunophenotypic changes included expression of CD7, CD11b, CD15, CD36, and CD56; CD34 overexpression; HLA-DR variability; lack of CD13 and CD33; underexpression of CD13, CD33, CD45, and HLA-DR; and partial loss of CD13, CD33, CD38, and CD117. In all cases, blasts were CD34+. Several blast immunophenotypic alterations are shared in neoplastic and nonneoplastic BM samples. Approximately 40% to 60% of neoplastic BM samples exhibited aberrancies not seen in reactive BM samples.
关于非急性髓系疾病的流式细胞术 (FC) 的数据受到可变门控策略的影响，并且对照仅限于正常骨髓 (BM) 样本。使用 4 色 FC 比较了骨髓增生异常综合征 (MDS)、骨髓增殖性肿瘤 (MPN) 和慢性髓单核细胞白血病 (CMML) 的诊断性 BM 样本中的原始细胞与 20 例非肿瘤性血细胞减少症/增多症 (CC) 和阴性分期 BM 样本。在 20 例 CC 中的 10 例中，原始细胞的免疫表型与对照样本存在差异。与对照样本相比，在 21 例 MDS 中的 18 例、14 例 MPN 中的 11 例和 7 例 CMML 中鉴定出免疫表型改变，而在 21 例 MDS 中的 13 例 (62%)、14 例 MPN 中的 7 例 (50%)和 7 例 CMML 中的 3 例 (43%)与 CC 相比。肿瘤特异性原始细胞免疫表型改变包括 CD7、CD11b、CD15、CD36 和 CD56 的表达；CD34 过表达；HLA-DR 变异性；缺乏 CD13 和 CD33；CD13、CD33、CD45 和 HLA-DR 表达减少；以及 CD13、CD33、CD38 和 CD117 的部分缺失。在所有情况下，原始细胞均为 CD34+。在肿瘤和非肿瘤性 BM 样本中存在几种原始细胞免疫表型改变。大约 40%至 60%的肿瘤性 BM 样本表现出与反应性 BM 样本中未见的异常。
