Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
Curr Microbiol. 2011 Mar;62(3):739-45. doi: 10.1007/s00284-010-9777-0. Epub 2010 Oct 21.
Chitosan is an attractive preparation widely used as a pharmaceutical excipient. This study aimed to evaluate the antimicrobial activities of chitosan derivatives, EDTA, and the newly developed chitosan-EDTA combination against Gram-negative and Gram-positive bacteria as well as Candida albicans. Antimicrobial activity was studied. Both minimal Inhibitory Concentrations (MIC) and minimal biocidal concentrations (MBC) were determined. Chitosan acetic acid recorded lower MIC values against Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans than those exhibited by EDTA. EDTA failed to have inhibitory activity against Enterococcus faecalis as well as MBC against any of the studied microorganisms. Chitosan acetic acid's MBC were recorded to all examined species. Checkerboard assay results indicated a synergistic antimicrobial activity of the new combination against Staphylococcus aureus and an additive effect against other microorganisms. Moreover, a short microbial exposure to chitosan-EDTA (20-30 min) caused complete eradication. Due to the continuous emergence of resistant strains, there is an urgent need to discover new antimicrobial agents. Our findings suggest the use of chitosan as an enhancing agent with antibacterial and antifungal properties in combination with EDTA in pharmaceutical preparations.
壳聚糖作为一种药用辅料被广泛应用，具有很大吸引力。本研究旨在评估壳聚糖衍生物、EDTA 和新开发的壳聚糖-EDTA 联合对革兰氏阴性和革兰氏阳性菌以及白色念珠菌的抗菌活性。研究了抗菌活性。测定了最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。与 EDTA 相比，壳聚糖乙酸盐对粪肠球菌、大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的 MIC 值较低。EDTA 对粪肠球菌没有抑制活性，对任何研究的微生物也没有 MBC。壳聚糖乙酸盐的 MBC 记录了所有被检查的物种。棋盘试验结果表明，新组合对金黄色葡萄球菌具有协同抗菌活性，对其他微生物具有相加作用。此外，微生物短时间接触壳聚糖-EDTA（20-30 分钟）可完全清除。由于耐药菌株的不断出现，迫切需要发现新的抗菌剂。我们的研究结果表明，壳聚糖可用作具有抗菌和抗真菌特性的增强剂，与 EDTA 联合用于药物制剂。
