Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Parasitol Res. 2011 Mar;108(3):687-91. doi: 10.1007/s00436-010-2114-5. Epub 2010 Oct 22.
The effectiveness of a bacteriocin-like substance (BLS) produced by Bacillus amyloliquefaciens was tested against Acanthamoeba polyphaga strains, and its cytotoxic potential on Vero cells was investigated. Amebicidal activity of the purified BLS was tested by plate bioassays with concentrations ranging from 12.5 to 6,400 AU mL(-1). Damage to A. pholyphaga cells was monitored using an inverted microscope and counted in a Fuchs-Rosenthal chamber after 24, 48, and 72 h. According to the results obtained, the BLS showed remarkable amebicidal and amebostatic effect on A. polyphaga and showed no cytotoxicity on the Vero cells. These results may have great relevance in the development of new acanthamoebicidal compounds.
检测了由解淀粉芽孢杆菌产生的细菌素样物质（BLS）对多形棘阿米巴原虫菌株的有效性，并研究了其对非洲绿猴肾细胞（Vero 细胞）的细胞毒性潜力。通过平板生物测定，用浓度范围为 12.5 至 6400 AU/mL 的方法测试了纯化 BLS 的杀阿米巴活性。在倒置显微镜下监测多形棘阿米巴细胞的损伤，并在 24、48 和 72 小时后用福氏罗氏计数室进行计数。根据获得的结果，BLS 对多形棘阿米巴显示出显著的杀阿米巴和阿米巴静止作用，对 Vero 细胞没有细胞毒性。这些结果可能对开发新的杀棘阿米巴化合物具有重要意义。
