Institut für Biologie/Bakteriengenetik, Humboldt-Universita¨t zu Berlin, 10115 Berlin, Germany.
J Bacteriol. 2011 Jan;193(1):215-24. doi: 10.1128/JB.00784-10. Epub 2010 Oct 22.
Here we report on a novel thiazole/oxazole-modified microcin (TOMM) from Bacillus amyloliquefaciens FZB42, a Gram-positive soil bacterium. This organism is well known for stimulating plant growth and biosynthesizing complex small molecules that suppress the growth of bacterial and fungal plant pathogens. Like microcin B17 and streptolysin S, the TOMM from B. amyloliquefaciens FZB42 undergoes extensive posttranslational modification to become a bioactive natural product. Our data show that the modified peptide bears a molecular mass of 1,335 Da and displays antibacterial activity toward closely related Gram-positive bacteria. A cluster of 12 genes that covers ∼10 kb is essential for the production, modification, export, and self-immunity of this natural product. We have named this compound plantazolicin (PZN), based on the association of several producing organisms with plants and the incorporation of azole heterocycles, which derive from Cys, Ser, and Thr residues of the precursor peptide.
我们在这里报告了一种来自解淀粉芽孢杆菌 FZB42 的新型噻唑/噁唑修饰型微菌素（TOMM），这是一种革兰氏阳性土壤细菌。该生物体以刺激植物生长和生物合成抑制细菌和真菌植物病原体生长的复杂小分子而闻名。与微菌素 B17 和链球菌溶血素 S 一样，来自解淀粉芽孢杆菌 FZB42 的 TOMM 经历了广泛的翻译后修饰，成为一种具有生物活性的天然产物。我们的数据表明，经过修饰的肽具有 1335 Da 的分子量，并对密切相关的革兰氏阳性细菌表现出抗菌活性。一个包含约 10 kb 的 12 个基因簇对于该天然产物的产生、修饰、外排和自身免疫至关重要。我们根据几个产生菌与植物的联系以及杂环唑的掺入，将这种化合物命名为植物霉素（PZN），杂环唑来源于前体肽的半胱氨酸、丝氨酸和苏氨酸残基。
