Department of Cosmetic Science and Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan, Republic of China.
J Nat Prod. 2010 Nov 29;73(11):1767-74. doi: 10.1021/np100281g. Epub 2010 Oct 25.
Antioxidant and antimelanogenesis activities of protocatechuic acid (1) from Origanum vulgare (oregano) were investigated. The antioxidative capacity of 1 was confirmed from its free-radical-scavenging activities, inhibition of lipid peroxidation, and suppression of reactive oxygen species in H(2)O(2)-induced BNLCL2 cells. The inhibition by 1 of tyrosinase and DOPA oxidase activity and melanin production was possibly related to the down-regulation of melanocortin-1 receptor, microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related proteins-2, and tyrosinase-related proteins-1 expression in α-melanocyte-stimulating hormone-induced B16 cells. After a gel containing 1 was applied to mice, the values of L* slightly increased, and a* and erythema-melanin levels of skin were reduced by comparing the values of untreated control groups, indicating 1 can reduce melanin production. These results suggest that 1 may act as an effective quencher of oxidative attackers with antimelanogenesis properties.
研究了迷迭香（oregano）中对苯二酚酸（1）的抗氧化和抗黑色素生成活性。1 的自由基清除活性、抑制脂质过氧化和抑制 H 2 O 2 诱导的 BNLCL2 细胞中活性氧的能力证实了其抗氧化能力。1 对酪氨酸酶和多巴氧化酶活性和黑色素生成的抑制可能与黑色素皮质素-1 受体、小眼相关转录因子、酪氨酸酶、酪氨酸酶相关蛋白-2 和酪氨酸酶相关蛋白-1 在α-黑色素细胞刺激素诱导的 B16 细胞中的下调有关。在将含有 1 的凝胶应用于小鼠后，与未处理的对照组相比，L值略有增加，a和红斑黑色素水平降低，这表明 1 可以减少黑色素的生成。这些结果表明，1 可能是一种有效的抗氧化剂，具有抗黑色素生成特性。
