• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

IMP-13 金属β-内酰胺酶的纯化和生化特性分析。

Purification and biochemical characterization of IMP-13 metallo-beta-lactamase.

机构信息

Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

出版信息

Antimicrob Agents Chemother. 2011 Jan;55(1):399-401. doi: 10.1128/AAC.00421-10. Epub 2010 Oct 25.

DOI:10.1128/AAC.00421-10
PMID:20974864
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3019620/
Abstract

The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.

摘要

IMP-13 金属β-内酰胺酶在大肠杆菌 BL21(DE3)中过表达，并通过色谱法进行纯化。动力学参数分析显示与其他 IMP 型酶存在一些显著差异，值得注意的是，对替卡西林和哌拉西林的催化效率更高，对亚胺培南的偏好明显高于美罗培南。

相似文献

1
Purification and biochemical characterization of IMP-13 metallo-beta-lactamase.IMP-13 金属β-内酰胺酶的纯化和生化特性分析。
Antimicrob Agents Chemother. 2011 Jan;55(1):399-401. doi: 10.1128/AAC.00421-10. Epub 2010 Oct 25.
2
Protection of piperacillin and ticarcillin from beta-lactamase hydrolysis by tazobactam (YTR-830) and clavulanic acid.
J Antimicrob Chemother. 1989 Jul;24(1):89-92. doi: 10.1093/jac/24.1.89.
3
Purification and biochemical characterization of the VIM-1 metallo-beta-lactamase.VIM-1金属β-内酰胺酶的纯化及生化特性研究
Antimicrob Agents Chemother. 2000 Nov;44(11):3003-7. doi: 10.1128/AAC.44.11.3003-3007.2000.
4
Plasmid-encoded metallo-beta-lactamase (IMP-6) conferring resistance to carbapenems, especially meropenem.质粒编码的金属β-内酰胺酶（IMP-6）赋予对碳青霉烯类抗生素，尤其是美罗培南的耐药性。
Antimicrob Agents Chemother. 2001 May;45(5):1343-8. doi: 10.1128/AAC.45.5.1343-1348.2001.
5
Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-beta-lactamase IMP-1 produced by Escherichia coli.由大肠杆菌产生的铜绿假单胞菌101/1477金属β-内酰胺酶IMP-1的生化特性
Antimicrob Agents Chemother. 1999 Apr;43(4):902-6. doi: 10.1128/AAC.43.4.902.
6
Identification of 76 novel B1 metallo-β-lactamases through large-scale screening of genomic and metagenomic data.通过大规模筛选基因组和宏基因组数据鉴定 76 种新型 B1 金属β-内酰胺酶。
Microbiome. 2017 Oct 12;5(1):134. doi: 10.1186/s40168-017-0353-8.
7
Biochemical characterization of the POM-1 metallo-β-lactamase from Pseudomonas otitidis.来自耳炎假单胞菌的POM-1金属β-内酰胺酶的生化特性
Antimicrob Agents Chemother. 2015 Mar;59(3):1755-8. doi: 10.1128/AAC.03843-14. Epub 2014 Dec 15.
8
Phenotypic Characterization of Multidrug-resistant Escherichia Coli with Special Reference to Extended-spectrum-beta-lactamases and Metallo-beta-lactamases in a Tertiary Care Center.三级医疗中心耐多药大肠杆菌的表型特征，特别提及超广谱β-内酰胺酶和金属β-内酰胺酶
JNMA J Nepal Med Assoc. 2015 Apr-Jun;53(198):89-95.
9
Role of permeability in the activities of beta-lactams against gram-negative bacteria which produce a group 3 beta-lactamase.通透性在β-内酰胺类抗生素对产生3型β-内酰胺酶的革兰氏阴性菌活性中的作用。
Antimicrob Agents Chemother. 1999 Aug;43(8):2084-6. doi: 10.1128/AAC.43.8.2084.
10
Beta-lactamase production in members of the family Enterobacteriaceae and resistance to beta-lactam-enzyme inhibitor combinations.肠杆菌科细菌中β-内酰胺酶的产生及对β-内酰胺酶抑制剂联合制剂的耐药性。
Antimicrob Agents Chemother. 1990 Apr;34(4):622-7. doi: 10.1128/AAC.34.4.622.

引用本文的文献

1
The synergistic effect of essential oil and carvacrol with imipenem against carbapenem-resistant : , molecular docking, and molecular dynamics studies.精油和香芹酚与亚胺培南联合对耐碳青霉烯类细菌的协同作用：实验、分子对接和分子动力学研究
Front Pharmacol. 2025 May 22;16:1582102. doi: 10.3389/fphar.2025.1582102. eCollection 2025.
2
Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector.利用大肠杆菌作为表达宿主和 pET-28a 作为克隆载体表达和纯化可溶性重组β-内酰胺酶。
Microb Cell Fact. 2022 Nov 23;21(1):244. doi: 10.1186/s12934-022-01972-5.
3
Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-β-lactamases.IMP-6 和 IMP-1 金属β-内酰胺酶底物特异性的结构见解。
J Biochem. 2022 Dec 27;173(1):21-30. doi: 10.1093/jb/mvac080.
4
Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.金属β-内酰胺酶在多药耐药时代：从结构和机制到进化、传播和抑制剂设计。
Chem Rev. 2021 Jul 14;121(13):7957-8094. doi: 10.1021/acs.chemrev.1c00138. Epub 2021 Jun 15.
5
Structure and Molecular Recognition Mechanism of IMP-13 Metallo-β-Lactamase.IMP-13 金属β-内酰胺酶的结构与分子识别机制。
Antimicrob Agents Chemother. 2020 May 21;64(6). doi: 10.1128/AAC.00123-20.
6
B1-Metallo-β-Lactamases: Where Do We Stand?B1型金属β-内酰胺酶：我们目前的状况如何？
Curr Drug Targets. 2016;17(9):1029-50. doi: 10.2174/1389450116666151001105622.
7
Antimicrobial active herbal compounds against Acinetobacter baumannii and other pathogens.针对鲍曼不动杆菌和其他病原体的具有抗菌活性的草药化合物。
Front Microbiol. 2015 Jun 18;6:618. doi: 10.3389/fmicb.2015.00618. eCollection 2015.
8
Systematic analysis of metallo-β-lactamases using an automated database.利用自动化数据库对金属β-内酰胺酶进行系统分析。
Antimicrob Agents Chemother. 2012 Jul;56(7):3481-91. doi: 10.1128/AAC.00255-12. Epub 2012 Apr 30.

本文引用的文献

1
Mutational analysis of VIM-2 reveals an essential determinant for metallo-beta-lactamase stability and folding.VIM-2 的突变分析揭示了金属β-内酰胺酶稳定性和折叠的关键决定因素。
Antimicrob Agents Chemother. 2010 Aug;54(8):3197-204. doi: 10.1128/AAC.01336-09. Epub 2010 May 24.
2
Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues.革兰氏阴性细菌病原体中获得性碳青霉烯酶：检测和监测问题。
Clin Microbiol Infect. 2010 Feb;16(2):112-22. doi: 10.1111/j.1469-0691.2009.03116.x.
3
Full Resistance and decreased susceptibility to carbapenems in IMP-13-producing Pseudomonas aeruginosa isolates from an outbreak.在一次暴发中，产IMP-13的铜绿假单胞菌分离株对碳青霉烯类药物呈现完全耐药和敏感性降低。
Antimicrob Agents Chemother. 2010 Mar;54(3):1381-2. doi: 10.1128/AAC.00399-09. Epub 2010 Jan 4.
4
First organisms with acquired metallo-beta-lactamases (IMP-13, IMP-22, and VIM-2) reported in Austria.奥地利首次报告带有获得性金属β-内酰胺酶（IMP-13、IMP-22和VIM-2）的生物体。
Antimicrob Agents Chemother. 2009 May;53(5):2221-2. doi: 10.1128/AAC.01573-08. Epub 2009 Feb 17.
5
[Phenotypic and genotypic characterization of imipenem-resistant Pseudomonas aeruginosa isolated in a Buenos Aires hospital].[布宜诺斯艾利斯一家医院分离出的耐亚胺培南铜绿假单胞菌的表型和基因型特征]
Rev Argent Microbiol. 2008 Oct-Dec;40(4):238-45.
6
First countrywide survey of acquired metallo-beta-lactamases in gram-negative pathogens in Italy.意大利首次针对革兰氏阴性病原体中获得性金属β-内酰胺酶的全国性调查。
Antimicrob Agents Chemother. 2008 Nov;52(11):4023-9. doi: 10.1128/AAC.00707-08. Epub 2008 Sep 22.
7
[Detection of metallo-beta-lactamases in gram-negative bacilli isolated in hospitals from Romania--research fellowship report].[罗马尼亚医院分离出的革兰氏阴性杆菌中金属β-内酰胺酶的检测——研究奖学金报告]
Bacteriol Virusol Parazitol Epidemiol. 2007 Jan-Jun;52(1-2):45-9.
8
Carbapenemases: the versatile beta-lactamases.碳青霉烯酶：多功能β-内酰胺酶
Clin Microbiol Rev. 2007 Jul;20(3):440-58, table of contents. doi: 10.1128/CMR.00001-07.
9
Protein production by auto-induction in high density shaking cultures.通过高密度摇瓶培养中的自诱导进行蛋白质生产。
Protein Expr Purif. 2005 May;41(1):207-34. doi: 10.1016/j.pep.2005.01.016.
10
Italian metallo-beta-lactamases: a national problem? Report from the SENTRY Antimicrobial Surveillance Programme.意大利金属β-内酰胺酶：一个全国性问题？来自哨兵抗菌监测计划的报告。
J Antimicrob Chemother. 2005 Jan;55(1):61-70. doi: 10.1093/jac/dkh512. Epub 2004 Dec 1.