Department of Ophthalmology, Keio University School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Acta Ophthalmol. 2010 Nov;88(7):e259-64. doi: 10.1111/j.1755-3768.2010.01997.x. Epub 2010 Sep 9.
To investigate the amount of systemic oxidative stress-related DNA damage and serum total antioxidant status of patients with normal-tension glaucoma compared with healthy controls.
Forty-three patients with normal-tension glaucoma selected from 60 consecutive newly diagnosed patients with primary open-angle glaucoma were enrolled. Each patient's intraocular pressure (IOP) was measured seven times over a 24-hour period. Those whose highest IOP was over 21 mmHg were excluded. Forty control subjects were recruited. The serum total antioxidant status was assessed colorimetrically by its ability to reduce the generation of the radical cation ABTS (2,2-azinobis-3-ethylbenzthiazoline sulphonate). The urinary 8-hydroxy-2'-deoxyguanosine was measured as a marker of oxidative DNA damage and normalized to creatinine. The results were compared between healthy subjects and patients with normal-tension glaucoma.
The control and normal-tension glaucoma groups did now show significant differences for age, gender, serum fructosamine, cholesterol and triglyceride levels; systolic and diastolic blood pressure, body mass index; and prevalence of hypertension. The serum total antioxidant status was significantly higher (p = 0.01) and the urinary 8-hydroxy-2'-deoxyguanosine/creatinine level significantly lower (p = 0.03) in the patients with normal-tension glaucoma compared with the controls. Even after we adjusted the data for independent variables, the incidence of normal-tension glaucoma was significantly correlated with high serum total antioxidant levels (p = 0.03; odds ratio, 1.007; 95% confidence interval, 1.001-1.013) and low urinary 8-hydroxy-2'-deoxyguanosine/creatinine (p = 0.02; odds ratio 0.76; 95% confidence interval, 0.61-0.96).
Increased serum total antioxidant and decreased 8-hydroxy-2'-deoxyguanosine may reflect compensatory alterations in response to increased systemic oxidative stress in patients with normal-tension glaucoma.
研究正常眼压性青光眼患者与健康对照者之间与系统性氧化应激相关的 DNA 损伤量和血清总抗氧化状态。
从 60 例连续初诊的原发性开角型青光眼患者中选择 43 例正常眼压性青光眼患者。对每位患者的眼压进行 24 小时内 7 次测量，排除眼压最高超过 21mmHg 的患者。招募 40 名对照者。通过其还原 ABTS（2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸）自由基阳离子的能力来评估血清总抗氧化状态。将尿 8-羟基-2'-脱氧鸟苷作为氧化 DNA 损伤的标志物，并与肌酐进行归一化。将结果在健康受试者与正常眼压性青光眼患者之间进行比较。
对照组和正常眼压性青光眼组在年龄、性别、血清果糖胺、胆固醇和甘油三酯水平、收缩压和舒张压、体重指数以及高血压患病率方面无显著差异。与对照组相比，正常眼压性青光眼患者的血清总抗氧化状态显著较高（p=0.01），尿 8-羟基-2'-脱氧鸟苷/肌酐水平显著较低（p=0.03）。即使我们对独立变量的数据进行调整，正常眼压性青光眼的发生率仍与高血清总抗氧化水平显著相关（p=0.03；优势比，1.007；95%置信区间，1.001-1.013）和低尿 8-羟基-2'-脱氧鸟苷/肌酐（p=0.02；优势比，0.76；95%置信区间，0.61-0.96）。
血清总抗氧化水平升高和 8-羟基-2'-脱氧鸟苷降低可能反映了正常眼压性青光眼患者中系统性氧化应激增加的代偿性改变。
