年龄相关性黄斑变性中的氧化大分子损伤。
Oxidative macromolecular damage in age-related macular degeneration.
机构信息
Fatih University, Medical School, Department of Ophthalmology, Ankara, Turkey.
出版信息
Curr Eye Res. 2009 Dec;34(12):1089-93. doi: 10.3109/02713680903353772.
PURPOSE
To evaluate oxidative damage to the macromolecules, including protein, lipid, and DNA, in association with total oxidation status (TOS), total antioxidant capacity (TAC) in patients with exudative age-related macular degeneration (AMD).
MATERIALS AND METHODS
Serum levels of malondialdehyde (MDA) as an index of lipid peroxidation, protein carbonyl (PC) as a marker of protein oxidation, 8-hydroxy-29-deoxyguanosine (8-OHdG) as an indicator of oxidative DNA damage along with TOS, TAC were measured by specific methods in 47 patients with exudative AMD and 25 age- and sex-matched healthy subjects.
RESULTS
Significantly higher MDA, PC, 8-OHdG, TOS, and lower TAC levels were detected in the serum of patients with exudative AMD compared with their controls (p < 0.001).
CONCLUSION
The results indicate that an imbalance between TOS and TAC leads to not only increased lipid damage, but also protein and DNA damage. These first reported results suggest that protein and DNA damage might also play a role in the pathogenesis of AMD.
目的
评估与总氧化状态（TOS）和总抗氧化能力（TAC）相关的渗出性年龄相关性黄斑变性（AMD）患者的大分子（包括蛋白质、脂质和 DNA）的氧化损伤。
材料和方法
通过特定方法测量 47 例渗出性 AMD 患者和 25 名年龄和性别匹配的健康对照者的血清丙二醛（MDA）作为脂质过氧化的指标、蛋白羰基（PC）作为蛋白质氧化的标志物、8-羟基-2'-脱氧鸟苷（8-OHdG）作为氧化 DNA 损伤的指标，以及 TOS 和 TAC。
结果
与对照组相比，渗出性 AMD 患者的血清 MDA、PC、8-OHdG、TOS 水平显著升高，而 TAC 水平显著降低（p<0.001）。
结论
这些结果表明 TOS 和 TAC 之间的不平衡不仅导致脂质损伤增加，还导致蛋白质和 DNA 损伤。这些首次报道的结果表明，蛋白质和 DNA 损伤也可能在 AMD 的发病机制中发挥作用。
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