Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan, Republic of China.
J Nat Prod. 2010 Nov 29;73(11):1785-91. doi: 10.1021/np100401f. Epub 2010 Oct 27.
Eight new eunicellin-base diterpenoids, hirsutalins A-H (1-8), were isolated from the soft coral Cladiella hirsuta. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute configuration of 1 was determined by Mosher's method. Compounds 1, 5, and 6 have been shown to exhibit cytotoxicity toward several cancer cell lines. Compounds 2-4 and 8 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein, with compound 2 also effectively reducing the level of COX-2 protein.
从软珊瑚 Cladiella hirsuta 中分离得到了 8 个新的海兔毒素基二萜类化合物，分别命名为 hirsutalins A-H（1-8）。通过光谱方法，特别是 1D 和 2D NMR 实验，解析了它们的结构。通过 Mosher 方法确定了 1 的绝对构型。化合物 1、5 和 6 对几种癌细胞系表现出细胞毒性。化合物 2-4 和 8 被发现通过抑制 iNOS 蛋白的表达，在 LPS 刺激的 RAW264.7 巨噬细胞中具有显著的体外抗炎活性，其中化合物 2 还能有效降低 COX-2 蛋白的水平。
