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药用植物提取物抗捻转血矛线虫的药效。

Efficacy of anthelmintic properties of medicinal plant extracts against Haemonchus contortus.

机构信息

Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509, Vellore District, Tamil Nadu, India.

出版信息

Res Vet Sci. 2011 Dec;91(3):400-4. doi: 10.1016/j.rvsc.2010.09.018. Epub 2010 Oct 25.

Abstract

The development of anthelmintic resistance has made the search for alternatives to control gastrointestinal nematodes of small ruminants imperative. Among these alternatives are several medicinal plants traditionally used as anthelmintics. This present work evaluated the efficacy of ethyl acetate, acetone, and methanol dried leaf and seed extracts of five medicinal plants were tested in vitro ovicidal and larvicidal activities on Haemonchus contortus. The in vitro assay was based on egg hatch assay (EHA) and larval development assay (LDA), all plant extracts were evaluated at five concentrations 50, 25, 12.5, 6.25 and 3.13 mg/ml. The leaf and seed ethyl acetate, acetone and methanol of Annona squamosa, Eclipta prostrata, Solanum torvum, Terminalia chebula, and Catharanthus roseus extracts were showed complete inhibition (100%) at the maximum concentration tested (50 mg/ml). The overall findings of the present study have shown that our experimental plant extracts contain possible anthelmintic compounds.

摘要

驱虫剂耐药性的发展使得寻找替代方法来控制小反刍动物的胃肠道线虫变得至关重要。这些替代方法包括几种传统上用作驱虫剂的药用植物。本研究评估了乙酸乙酯、丙酮和甲醇干燥的五种药用植物的叶和种子提取物在体外对捻转血矛线虫的杀卵和杀幼虫活性。体外试验基于卵孵化试验(EHA)和幼虫发育试验(LDA),所有植物提取物均在五个浓度 50、25、12.5、6.25 和 3.13 mg/ml 下进行评估。番荔枝、犁头草、龙葵、诃子和长春花的叶和种子的乙酸乙酯、丙酮和甲醇提取物在测试的最大浓度(50mg/ml)下表现出完全抑制(100%)。本研究的总体结果表明,我们的实验植物提取物含有可能的驱虫化合物。

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