Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509 Vellore, Tamil Nadu, India.
Parasitol Res. 2011 Jul;109(1):37-45. doi: 10.1007/s00436-010-2218-y. Epub 2010 Dec 14.
The source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. The egg hatching and larvicidal effect of indigenous plant extracts were investigated against the sheep parasite, Haemonchus contortus. The purpose of the present study was to assess the efficacy of leaf, bark, and seed ethyl acetate, acetone and methanol extracts of Andrographis paniculata (Burm.f.) Wall. ex Nees., Anisomeles malabarica (L.) R. Br., Annona squamosa L., Datura metel L., and Solanum torvum Swartz were tested against the parasitic nematode of small ruminants H. contortus using egg hatch assay (EHA) and larval development assay (LDA). The assays were run in 24-well cell culture plates at room temperature with five replicates. All plant extracts showed moderate parasitic effects after 48 and exposure for egg hatching and LDA, respectively; however, 100% egg hatching and larvicidal inhibition were found in the methanol extracts of A. paniculata, A. squamosa, D. metel, and S. torvum at 25 mg/ml and the effect was similar to positive control of Albendazole (0.075 mg/ml) and Ivermectin (0.025mg/ml) against H. contortus, respectively. The EHA result showed the ED(50) of methanol extracts of A. paniculata and D. metel, which were 2.90 and 3.08 mg/ml, and in larval development assay, the ED(50) was 4.26 and 3.86 mg/ml, respectively. These effects remain to be confirmed through in vivo studies.
化学驱虫剂（左旋咪唑、氟苯达唑和噻苯达唑）的来源限制了绵羊和山羊胃肠道线虫病控制的成功，因此激发了人们对药用植物提取物作为驱虫剂替代来源的研究兴趣。本研究旨在评估穿心莲（Burm.f.）Wall. ex Nees.、马兜铃（L.）R. Br.、番荔枝（L.）、曼陀罗（L.）和龙葵（Swartz）叶、树皮和种子的乙酸乙酯、丙酮和甲醇提取物对绵羊寄生虫捻转血矛线虫的功效，采用卵孵化试验（EHA）和幼虫发育试验（LDA）进行了测试。在室温下，在 24 孔细胞培养板中进行了 5 个重复的试验。所有植物提取物在暴露于 EHA 和 LDA 分别孵育 48 小时后，均显示出中度的寄生虫效应；然而，在 25mg/ml 下，穿心莲、番荔枝、曼陀罗和龙葵的甲醇提取物中发现了 100%的卵孵化和幼虫抑制作用，其效果与阳性对照阿苯达唑（0.075mg/ml）和伊维菌素（0.025mg/ml）对捻转血矛线虫的效果相似。EHA 结果显示，穿心莲和曼陀罗甲醇提取物的 ED50 分别为 2.90 和 3.08mg/ml，在幼虫发育试验中，ED50 分别为 4.26 和 3.86mg/ml。这些效果仍需通过体内研究来证实。
