El-Beltagy Abd El-Fattah B M, Elsyyad Hassan I H, Abdelaziz Karoline K, Madany Amira S, Elghazaly Mohamed M
Zoology Department, Faculty of Science, Damanhour University, Damanhour, Egypt.
Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Breast Cancer (Dove Med Press). 2021 Jul 8;13:431-445. doi: 10.2147/BCTT.S306971. eCollection 2021.
To evaluate the potential therapeutic role of (graviola) fruit and bee venom (BV) against -methylnitrosourea (MNU)-induced breast cancer in pregnant female rats and complications in the ovaries.
A total of 24 female rats were induced with a single dose of MNU (50 mg/kg body weight). After confirmation of positive tumor marker, female rats were placed with the males for mating. The pregnant rats were randomly divided into four groups (n=6): MNU-induced only (group 1), MNU-induced rats and supplemented with 200 mg/kg diet (group 2), MNU-induced and treated with two doses of BV 75 μg/kg (group 3), and MNU-induced and treated with both and BV (group 4).
In group 1, the breast tissue of mothers revealed pronounced cellular hyperplasia and histopathological signs. Also, the ovarian tissue of mothers and their offspring displayed deleterious histological changes. In groups 2 and 4, histopathological signs and cellular hyperplasia markedly disappeared in breast tissue. However, the histopathological signs induced by MNU in the ovarian tissue reversed to normal in groups 2-4. Also in groups 2-4, levels of serum MMP1, NFκB, and TNFα significantly decreased, and serum caspase 3 significantly increased either in mother rats or their offspring compared to the MNU-alone group. Levels of serum MDA significantly decreased; however, levels of serum antioxidants (CAT and SOD) significantly increased in all groups 2-4 compared to MNU-alone group.
has a more powerful therapeutic role than BV against MNU-induced breast cancer in rats; however, both have a powerful ameliorative role against ovarian histopathological alterations induced by MNU. Such ameliorative effects of and BV are mainly attributed to their antioxidant, anti-inflammatory, and antiproliferative constituents.
评估番荔枝（巴西番荔枝）果实和蜂毒（BV）对N-甲基亚硝基脲（MNU）诱导的孕鼠乳腺癌及卵巢并发症的潜在治疗作用。
总共24只雌性大鼠接受单剂量MNU（50毫克/千克体重）诱导。在确认肿瘤标志物呈阳性后，将雌性大鼠与雄性大鼠合笼交配。将孕鼠随机分为四组（每组n = 6）：仅MNU诱导组（第1组）、MNU诱导且饮食中补充200毫克/千克番荔枝的大鼠组（第2组）、MNU诱导并用两剂75微克/千克BV治疗的大鼠组（第3组）、MNU诱导并用番荔枝和BV治疗的大鼠组（第4组）。
在第1组中，母鼠的乳腺组织显示出明显的细胞增生和组织病理学征象。此外，母鼠及其后代的卵巢组织呈现出有害的组织学变化。在第2组和第4组中，乳腺组织中的组织病理学征象和细胞增生明显消失。然而，MNU在卵巢组织中诱导的组织病理学征象在第2 - 4组中恢复正常。同样在第2 - 4组中，与仅MNU组相比，母鼠或其后代血清中基质金属蛋白酶1（MMP1）、核因子κB（NFκB）和肿瘤坏死因子α（TNFα）水平显著降低，血清半胱天冬酶3（caspase 3）显著升高。血清丙二醛（MDA）水平显著降低；然而，与仅MNU组相比，所有第2 - 4组血清抗氧化剂（过氧化氢酶（CAT）和超氧化物歧化酶（SOD））水平显著升高。
在对抗MNU诱导的大鼠乳腺癌方面，番荔枝比蜂毒具有更强的治疗作用；然而，二者在对抗MNU诱导的卵巢组织病理学改变方面均具有强大的改善作用。番荔枝和蜂毒的这种改善作用主要归因于它们的抗氧化、抗炎和抗增殖成分。
