Hasselaar P, Triplett D A, LaRue A, Derksen R H, Blokzijl L, de Groot P G, Wagenknecht D R, McIntyre J A
Department of Haematology, University Hospital Utrecht, The Netherlands.
J Rheumatol. 1990 Feb;17(2):186-91.
We found that levels of antiphospholipid antibodies (aPLA), measured with an ELISA increase if serum or plasma samples are heated. The phenomenon is dependent on duration and degree of heating, optimum levels being reached at 3 h at 56 degrees C. Negative samples become positive after heating. The heating effect is more pronounced for IgG-aPLA than for IgM-aPLA and is not observed for adsDNA, atetanus or lymphocytotoxic antibodies. The presence of serum/plasma components in addition to IgG is essential for the phenomenon to occur. Ultracentrifugation and mixing experiments with isolated IgG did not enable us to explain our observations. Nevertheless, knowledge of this phenomenon is of practical importance.
我们发现，用酶联免疫吸附测定法（ELISA）检测时，如果血清或血浆样本被加热，抗磷脂抗体（aPLA）水平会升高。这种现象取决于加热的持续时间和程度，在56摄氏度下加热3小时可达到最佳水平。阴性样本加热后会变为阳性。IgG-aPLA的加热效应比IgM-aPLA更明显，而对于抗双链DNA抗体、抗破伤风抗体或淋巴细胞毒性抗体则未观察到这种效应。除IgG外，血清/血浆成分的存在对于该现象的发生至关重要。超速离心和用分离出的IgG进行的混合实验无法解释我们的观察结果。然而，了解这一现象具有实际重要性。
