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根癌土壤杆菌A281在大豆和苜蓿植株上诱发的肿瘤中的Ti质粒DNA和冠瘿碱合成位点

T-DNA and opine synthetic loci in tumors incited by Agrobacterium tumefaciens A281 on soybean and alfalfa plants.

作者信息

Hood E E, Chilton W S, Chilton M D, Fraley R T

出版信息

J Bacteriol. 1986 Dec;168(3):1283-90. doi: 10.1128/jb.168.3.1283-1290.1986.

DOI:10.1128/jb.168.3.1283-1290.1986
PMID:3023301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213635/
Abstract

We report here the molecular characterization of transferred DNA (T-DNA) in leguminous tumors incited by Agrobacterium tumefaciens A281 harboring the tumor-inducing plasmid pTiBo542. The T-DNA is composed of two regions named TL (left portion)-DNA and TR (right portion)-DNA, in accordance with the nomenclature for the octopine strains. TL-DNA is defined by several internal HindIII restriction fragments totaling 10.8 kilobase pairs (kbp) in uncloned soybean and alfalfa tumors. Alfalfa tumor DNA may contain one more HindIII fragment at the left end of TL-DNA than does soybean tumor DNA. TR-DNA has a 5.8-kbp BamHI-EcoRI internal fragment. All borders other than the left border of TL-DNA appear to be the same within the detection limits of Southern blot hybridization experiments. The two T-DNA regions are separated by 16 to 19 kbp of DNA not stably maintained in tumors. The distance from the left border of TL-DNA to the right border of TR-DNA is approximately 40 kbp. Loci for the mannityl opines are situated in TR-DNA, based on genetic and biochemical criteria.

摘要

我们在此报告了由携带致瘤质粒pTiBo542的根癌土壤杆菌A281诱导的豆科植物肿瘤中转基因DNA（T-DNA）的分子特征。根据章鱼碱型菌株的命名法，T-DNA由两个区域组成，分别命名为TL（左部分）-DNA和TR（右部分）-DNA。在未克隆的大豆和苜蓿肿瘤中，TL-DNA由几个内部HindIII限制性片段组成，总计10.8千碱基对（kbp）。苜蓿肿瘤DNA在TL-DNA左端可能比大豆肿瘤DNA多一个HindIII片段。TR-DNA有一个5.8-kbp的BamHI-EcoRI内部片段。在Southern印迹杂交实验的检测限度内，除TL-DNA的左边界外，所有边界似乎都相同。两个T-DNA区域被16至19 kbp不稳定存在于肿瘤中的DNA隔开。从TL-DNA的左边界到TR-DNA的右边界的距离约为40 kbp。根据遗传和生化标准，甘露糖碱的基因座位于TR-DNA中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/6a8511b2f350/jbacter00205-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/8d026319d56c/jbacter00205-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/d045bb3e1b35/jbacter00205-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/6a8511b2f350/jbacter00205-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/8d026319d56c/jbacter00205-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/d045bb3e1b35/jbacter00205-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d41/213635/6a8511b2f350/jbacter00205-0248-a.jpg

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