Stela R, Berceanu S, Munteanu N, Ursea C, Motoiu I, Moraru I
Clinic of Hematology Fundeni Hospital, Bucharest.
Haematologia (Budap). 1990;23(4):211-21.
The study has been performed on peripheral blood and splenic malignant cells from 16 patients with hairy cell leukaemia (HCL). The cell surface markers were identified by rosette techniques and using monoclonal antibodies (m Ab). The surface markers' expression of the hairy cells (HC) varied. The E receptors, the T-cell antigens, the HLA-DR antigens and smIgG were either expressed or not according to the affected organ, the progress of illness, or the treatment. The surface pattern changed sometimes in the same patient during the progress of illness. These observations demonstrate that HCL is a unique disease with malignant cells characterized by a marked variability of the cell surface markers. To demonstrate the ability of hairy cells to bind labile smIgG, the cells were studied by affinity chromatography on SpA-Sepharose 6MB and by ES-rosette assay. The percent of cells bound on SpA-Sepharose varied between 6% and 66%, representing the hairy cells with labile-bound smIgG. With affinity chromatography it was also possible to separate the hairy cells with a special phenotype: T3+ T4+ T8+ T11+ surface membrane labile-bound IgG+ (11gG+) FcR+, HLA-DR+ EACD+ (Ripley rosette forming cells), resembling a normal subset of large granular lymphocytes (LGL). The percentage of these cells varied between 60% and 86% of the bound cells. These observations suggest that in HCL, the malignant transformation might involve a common progenitor for the B, T and LGL lineages, the hairy cell being a hybrid type of malignant cell. Its main immunological peculiarity is the marked mobility of the surface membrane structures and hence the lability (plasticity) of the surface markers' expression.
该研究对16例毛细胞白血病（HCL）患者的外周血和脾脏恶性细胞进行了检测。通过玫瑰花结技术和使用单克隆抗体（m Ab）鉴定细胞表面标志物。毛细胞（HC）的表面标志物表达各不相同。E受体、T细胞抗原、HLA - DR抗原和smIgG的表达与否取决于受累器官、疾病进展或治疗情况。在疾病进展过程中，同一患者的表面模式有时会发生变化。这些观察结果表明，HCL是一种独特的疾病，其恶性细胞的特征是细胞表面标志物具有显著变异性。为了证明毛细胞结合不稳定smIgG的能力，通过在SpA - Sepharose 6MB上进行亲和层析和ES - 玫瑰花结试验对细胞进行了研究。结合在SpA - Sepharose上的细胞百分比在6%至66%之间，代表结合有不稳定smIgG的毛细胞。通过亲和层析还可以分离出具有特殊表型的毛细胞：T3 + T4 + T8 + T11 + 表面膜不稳定结合IgG + （11gG + ）FcR + ，HLA - DR + EACD + （形成瑞普利玫瑰花结的细胞），类似于大颗粒淋巴细胞（LGL）的正常亚群。这些细胞的百分比在结合细胞的60%至86%之间。这些观察结果表明，在HCL中，恶性转化可能涉及B、T和LGL谱系的共同祖细胞，毛细胞是一种恶性细胞的杂交类型。其主要免疫特性是表面膜结构的显著流动性，因此表面标志物表达具有不稳定性（可塑性）。
