Williams M E, Mundy J, Kay S A, Chua N H
Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021.
Plant Mol Biol. 1990 May;14(5):765-74. doi: 10.1007/BF00016509.
We have screened a pea genomic library using a cDNA probe derived from pea shoot RNA. From this screen, we isolated two closely related genes, designated as S2 and P4. An intriguing property of these two genes is the presence in their coding region of a repeated sequence that is conserved between them in sequence but not in the number of the repeating units. The predicted amino acid sequence suggests that these proteins could be exported and glycosylated. 3' S1 analysis reveals that one of the genes, S2, is expressed highly in stem, as expected from previous work. However, mRNA derived from the other gene, P4, is not detectable in stem tissue, but is present in tissue derived from pea pods. The 5' upstream sequence of S2 and P4 are 94% identical up to position -121, suggesting that sequences upstream of -121 are responsible for organ-specific expression of the two genes.
我们使用从豌豆茎RNA衍生而来的cDNA探针筛选了豌豆基因组文库。通过此次筛选，我们分离出了两个紧密相关的基因，命名为S2和P4。这两个基因的一个有趣特性是，在它们的编码区域存在一个重复序列，该序列在它们之间序列保守，但重复单元的数量不同。预测的氨基酸序列表明这些蛋白质可能会被输出并进行糖基化。3' S1分析表明，其中一个基因S2如先前工作所预期的那样在茎中高度表达。然而，来自另一个基因P4的mRNA在茎组织中无法检测到，但存在于豌豆豆荚衍生的组织中。S2和P4的5'上游序列在-121位置之前94%相同，这表明-121位置上游的序列负责这两个基因的器官特异性表达。
