植物叶绿体中的密码子识别机制。
Codon recognition mechanisms in plant chloroplasts.
作者信息
Pfitzinger H, Weil J H, Pillay D T, Guillemaut P
机构信息
Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
出版信息
Plant Mol Biol. 1990 May;14(5):805-14. doi: 10.1007/BF00016513.
In chloroplasts, all 61 sense codons are found in chloroplast (cp) DNA sequences coding for proteins. However among the sequenced cp tRNAs or tRNA genes, tRNAs with anticodons complementary to codons CUU/C (Leu), CCU/C (Pro), GCU/C (Ala) and CGC/A/G (Arg) [or CGC/A (Arg) in Marchantia] have not been found. In this paper we show that cp tRNA(Ala)(UGC) cp tRNA(Pro)(UGG) and cp tRNA(Arg)(ICG) are able to decode the corresponding four-codon family. In the case of leucine codons CUU/C, we show that 'U:U and U:C wobble' mechanisms can operate to allow the reading of these codons by cp tRNA(Leu)(UAm7G).
在叶绿体中，编码蛋白质的叶绿体（cp）DNA序列中存在所有61种有义密码子。然而，在已测序的cp tRNA或tRNA基因中，尚未发现其反密码子与密码子CUU/C（亮氨酸）、CCU/C（脯氨酸）、GCU/C（丙氨酸）和CGC/A/G（精氨酸）[在地钱中为CGC/A（精氨酸）]互补的tRNA。在本文中，我们表明cp tRNA（Ala）（UGC）、cp tRNA（Pro）（UGG）和cp tRNA（Arg）（ICG）能够解码相应的四密码子家族。对于亮氨酸密码子CUU/C，我们表明“U:U和U:C摆动”机制可以发挥作用，使cp tRNA（Leu）（UAm7G）能够读取这些密码子。
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