从椭圆小球藻中分离和鉴定叶绿体 DNA。
Isolation and Characterization of Chloroplast DNA from Chlorella ellipsoidea.
机构信息
Mitsubishi-Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194, Japan.
出版信息
Plant Physiol. 1982 Jul;70(1):92-6. doi: 10.1104/pp.70.1.92.
A circular DNA molecule was isolated from chloroplasts of Chorella ellipsoidea. The DNA had a buoyant density of 1.695 grams per cubic centimeter (36% GC) and a contour length of 56 micrometers (175 kilobase pairs). The restriction endonuclease analysis gave the same size. Agarose gel electrophoretic patterns of chloroplast DNA digested by several restriction endonucleases were also presented. The digestion by the restriction enzymes, HpaII, MspI, SmaI, and XmaI revealed no appreciable methylation at CG sites in chloroplast DNA.
从椭圆小球藻的叶绿体中分离出一个环状 DNA 分子。该 DNA 的浮力密度为每立方厘米 1.695 克（GC 含量 36%），周长为 56 微米（175 千碱基对）。限制内切酶分析也给出了相同的大小。还展示了几种限制内切酶消化叶绿体 DNA 的琼脂糖凝胶电泳图谱。用限制酶 HpaII、MspI、SmaI 和 XmaI 进行消化，未发现叶绿体 DNA 中 CG 位点有明显的甲基化。
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