Departmento de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP)-São Paulo/SP, Brazil.
Lipids Health Dis. 2010 Oct 30;9:125. doi: 10.1186/1476-511X-9-125.
Sleep deprivation has been shown to increase inflammatory markers in rat sera and peripheral blood mononuclear cells. Inflammation is a condition associated with pathologies such as obesity, cancer, and cardiovascular diseases. We investigated changes in the pro and anti-inflammatory cytokines and adipokines in different depots of white adipose tissue in rats. We also assessed lipid profiles and serum levels of corticosterone, leptin, and adiponectin after 96 hours of sleep deprivation.
The study consisted of two groups: a control (C) group and a paradoxical sleep deprivation by 96 h (PSD) group. Ten rats were randomly assigned to either the control group (C) or the PSD. Mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue, liver and serum were collected following completion of the PSD protocol. Levels of interleukin (IL)-6, interleukin (IL)-10 and tumour necrosis factor (TNF)-α were analysed in MEAT and RPAT, and leptin, adiponectin, glucose, corticosterone and lipid profile levels were analysed in serum.
IL-6 levels were elevated in RPAT but remained unchanged in MEAT after PSD. IL-10 protein concentration was not altered in either depot, and TNF-α levels decreased in MEAT. Glucose, triglycerides (TG), VLDL and leptin decreased in serum after 96 hours of PSD; adiponectin was not altered and corticosterone was increased.
PSD decreased fat mass and may modulate the cytokine content in different depots of adipose tissue. The inflammatory response was diminished in both depots of adipose tissue, with increased IL-6 levels in RPAT and decreased TNF-α protein concentrations in MEAT and increased levels of corticosterone in serum.
睡眠剥夺已被证明会增加大鼠血清和外周血单核细胞中的炎症标志物。炎症是与肥胖、癌症和心血管疾病等病理学相关的一种状况。我们研究了不同白色脂肪组织储存部位促炎和抗炎细胞因子和脂肪因子的变化。我们还评估了 96 小时睡眠剥夺后大鼠的血脂谱和血清皮质酮、瘦素和脂联素水平。
该研究包括两个组：对照组（C）和通过 96 小时进行的反常睡眠剥夺组（PSD）。将 10 只大鼠随机分配到对照组（C）或 PSD 组。完成 PSD 方案后，收集肠系膜（MEAT）和腹膜后（RPAT）脂肪组织、肝脏和血清。分析 MEAT 和 RPAT 中的白细胞介素（IL）-6、白细胞介素（IL）-10 和肿瘤坏死因子（TNF）-α水平，并分析血清中的瘦素、脂联素、葡萄糖、皮质酮和血脂谱水平。
PSD 后 RPAT 中的 IL-6 水平升高，但 MEAT 中的 IL-10 蛋白浓度没有变化，且 MEAT 中的 TNF-α水平降低。96 小时 PSD 后血清中的葡萄糖、甘油三酯（TG）、VLDL 和瘦素降低；脂联素没有改变，皮质酮增加。
PSD 减少了脂肪量，并可能调节不同脂肪组织储存部位的细胞因子含量。在两个脂肪组织储存部位中，炎症反应均减弱，RPAT 中 IL-6 水平升高，MEAT 中 TNF-α蛋白浓度降低，血清中皮质酮水平升高。
