Weiss Alexander Th A, Klopfleisch Robert, Gruber Achim D
Department of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str 15, 14163 Berlin, Germany.
J Feline Med Surg. 2010 Dec;12(12):929-35. doi: 10.1016/j.jfms.2010.07.006. Epub 2010 Oct 30.
A significant drop in the prevalence of feline leukaemia virus (FeLV) antigenaemic cats and antigen-associated lymphomas has been observed after the introduction of FeLV vaccination and antigen-testing with removal of persistently antigenaemic cats. However, recent reports have indicated that regressively infected cats may contain FeLV provirus DNA and that lymphoma development may be associated with the presence of provirus alone. In the present study, we investigated the presence of FeLV antigen and provirus DNA in 50 lymphomas by immunohistochemistry and semi-nested polymerase chain reaction, respectively. Interestingly, almost 80% of T-cell lymphomas and 60% of B-cell lymphomas contained provirus DNA while only 21% of T-cell lymphomas and 11% of B-cell lymphomas expressed FeLV antigen. In conclusion, our results support previous hypotheses that vaccination and removal of persistently antigenaemic cats have led to a drop in FeLV antigen-expressing lymphomas. However, FeLV provirus DNA is still present in a high percentage of feline lymphomas.
在引入猫白血病病毒（FeLV）疫苗接种以及通过检测清除持续抗原血症猫的抗原检测后，已观察到抗原血症猫和抗原相关淋巴瘤的患病率显著下降。然而，最近的报告表明，隐性感染的猫可能含有FeLV前病毒DNA，并且淋巴瘤的发展可能仅与前病毒的存在有关。在本研究中，我们分别通过免疫组织化学和半巢式聚合酶链反应调查了50例淋巴瘤中FeLV抗原和前病毒DNA的存在情况。有趣的是，几乎80%的T细胞淋巴瘤和60%的B细胞淋巴瘤含有前病毒DNA，而只有21%的T细胞淋巴瘤和11%的B细胞淋巴瘤表达FeLV抗原。总之，我们的结果支持了先前的假设，即疫苗接种和清除持续抗原血症猫导致了表达FeLV抗原的淋巴瘤的减少。然而，FeLV前病毒DNA在猫淋巴瘤中仍有很高的比例存在。
